I Stat Creatinine Analysis
I Stat Creatinine Analysis - Related Videos
11:19
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Cited by 2 •
2016
Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.
08:42
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
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Cited by 4 •
2020
This protocol describes a canonical method to understand the critical genes controlling osteoclast activity in vivo. This method uses a transgenic mouse model and some canonical techniques to analyze skeletal phenotype.
01:25
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance
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2025
In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance (Clcr), a...
09:40
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
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2017
DNA-protein interactions are essential for multiple biological processes. During the evaluation of cellular functions, the analysis of DNA-protein interactions is indispensable for understanding gene regulation. Chromatin immunoprecipitation (ChIP) is a powerful tool to analyze such interactions in vivo.
01:28
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration
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2025
Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area. This equation is...
FAQs
The cartridge couples chemistry to detection in a defined sequence. Enzymatic reactions first convert creatinine through intermediate compounds, then generate hydrogen peroxide. An electrochemical sensor detects that product, and the system uses the measured signal to calculate creatinine concentration. This coupling allows biochemical conversion to become a quantitative result.
Whole blood is the specimen analyzed by the assay and is handled within the cartridge alongside the reaction chemistry and sensor measurement. Integrating these functions in one portable platform supports rapid access to renal-function information at the point of care, without separating sample handling from the analytical process.
Creatinine concentration serves as an indicator of kidney filtration and renal function, so the measured value has biological meaning beyond the sensor signal. A result can support assessment of kidney performance and help identify impaired filtration. The analysis therefore links a molecular measurement in blood with an interpretation about renal physiology.
A basic i-STAT creatinine analysis workflow combines sample handling, reaction chemistry, and electrochemical measurement. Whole blood is processed in the cartridge, enzymatic steps generate hydrogen peroxide, and the sensor detects the resulting signal. The instrument then calculates creatinine concentration, producing data when immediate information is needed and laboratory testing is not readily available.
Point-of-care use is most relevant when clinicians need kidney-related information promptly and laboratory testing is not immediately available. In that setting, the portable system can support evaluation of kidney performance, recognition of potentially impaired filtration, and clinical decision-making. Its value comes from combining rapid access with a biologically meaningful measurement.
In biology, the method exemplifies analytical biology: a biochemical reaction is engineered into a measurement system that reports patient data. The enzymatic conversion supplies a detectable chemical product, while electrochemical sensing translates that product into concentration. This design demonstrates how molecular events can be integrated with portable instrumentation for clinically useful renal assessment.