In Situ Remediation
In Situ Remediation - Related Videos
05:40
Comparison of Scale in a Photosynthetic Reactor System for Algal Remediation of Wastewater
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Cited by 3 •
2017
An experimental methodology is presented to compare the performance of small (100 L) and large (1,000 L) scale reactors designed for algae remediation of landfill wastewater. System characteristics, including surface area to volume ratio, retention time, biomass density, and wastewater feed concentrations, can be adjusted based on application.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
08:32
Ultrasound Images of the Tongue: A Tutorial for Assessment and Remediation of Speech Sound Errors
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Cited by 41 •
2017
Ultrasound imaging can be used to display the shape and movements of the tongue in real time during speech. The images can be used to determine the nature of speech sound errors. Visual feedback of the tongue can be used to facilitate improvements in speech sound production in clinical populations.
09:39
Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites
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Cited by 26 •
2014
Biochar is a carbon-rich material used as a soil amendment with the ability to sustainably sequester carbon, improve substrate quality and sorb contaminants. This protocol describes the 17 analytical methods used for the characterization of biochar, which is required prior to large scale implementation of these amendments in the environment.
08:30
A Complete Method for Evaluating the Performance of Photocatalysts for the Degradation of Antibiotics in Environmental Remediation
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Cited by 5 •
2022
Presented here is a protocol to explore a universal set of experimental procedures for comprehensive laboratory evaluation of photocatalysts in the field of environmental purification, using the example of photocatalytic removal of antibiotic organic pollutant molecules from water by phthalocyanine sensitized silver phosphate composites.
FAQs
Amendments shape microbial metabolism by supplying nutrients, electron donors, or electron acceptors that may be lacking in the contaminated environment. These additions can promote microbial activity and support transformation or degradation of pollutants. Their value depends on whether they reach the affected zone and create conditions compatible with the organisms responsible for the desired treatment process.
Microbial communities do not respond uniformly across a site. Their activity is influenced by subsurface conditions and by the availability and distribution of treatment agents. Consequently, an amendment can be suitable yet ineffective if it does not contact the relevant microbial populations or if local conditions do not support their metabolism. Site-specific evaluation is therefore essential.
Contaminant properties help determine whether biological transformation or degradation is feasible and which treatment conditions matter most. In situ remediation can address petroleum compounds, chlorinated solvents, and other contaminants, but the same amendment strategy should not be assumed to work for every pollutant. Matching the contaminant with appropriate microbial activity and subsurface conditions guides treatment design.
Monitoring is needed to distinguish actual cleanup progress from simple changes in contaminant distribution. Measurements over time can be interpreted alongside information about microbial communities, subsurface conditions, and amendment distribution. This evidence helps determine whether treatment is promoting pollutant transformation or degradation and whether environmental recovery is occurring, rather than relying on amendment application alone.
A biological in situ remediation program begins with evaluating the contaminants, microbial communities, and subsurface conditions. Researchers then select amendments that provide needed nutrients, electron donors, or electron acceptors and consider how those agents will distribute through the affected area. Continued monitoring assesses cleanup progress and environmental recovery, allowing performance to be judged under actual site conditions.
This approach is useful when limiting excavation, removal, site disturbance, and waste transport is important. Its biological basis makes it relevant to studies of microbial metabolism and environmental recovery, especially at sites containing petroleum compounds or chlorinated solvents. Researchers must still account for contaminant properties and site variability, because treatment effectiveness depends on more than selecting a pollutant category.