Intermediary Metabolism
Intermediary Metabolism - Related Videos
12:10
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
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Cited by 12 •
2011
Stable isotopic profiling by gas chromatography mass spectrometric analysis of intermediary metabolic flux is described in the nematode, Caenorhabditis elegans. Methods are detailed for assessing isotopic enrichment in carbon dioxide, organic acids, and amino acids following isotope exposure either during development on agar plates or during adulthood in liquid culture.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
These pathways are interconnected rather than isolated. Glycolysis, the citric acid cycle, and amino acid and lipid metabolism exchange metabolic intermediates, allowing nutrients to contribute to energy production or cellular construction. This organization lets cells redirect available materials toward ATP generation, precursor supply, or waste formation according to current physiological demands.
Cellular survival requires more than energy alone. Intermediary metabolism must generate ATP for cellular work while also preserving or producing precursors needed for growth, repair, and macromolecule synthesis. The balance changes with nutrient availability and energy demand, so pathway activity is adjusted rather than maintained at a fixed level.
ATP and NADH represent distinct products of metabolic activity. ATP provides the energy that powers cellular work, while NADH is a reducing equivalent generated alongside other metabolic outputs. Considering both helps explain how pathways support immediate energy needs while also producing chemically useful reducing capacity within the cell.
Regulatory signals adjust metabolic flux, meaning the movement of material through interconnected biochemical pathways. When cellular energy demands or nutrient availability change, these signals can shift pathway activity toward greater energy generation, precursor production, or metabolic waste formation. This coordination prevents the network from operating independently of the cell’s current requirements.
The framework connects nutrient availability and physiological demand with changes in pathway activity. Nutrition alters the materials entering metabolic pathways, whereas exercise changes cellular energy requirements. Examining how flux responds to these conditions can clarify whether metabolism emphasizes ATP production, precursor availability, or other outputs needed to support cellular function.
Disruptions in enzymes or pathway regulation can change how nutrients are converted into ATP, reducing equivalents, cellular building blocks, or waste. Studying these changes provides a biochemical context for inherited metabolic disorders and disease-associated alterations. The same pathway framework also helps relate molecular changes to effects on cell survival, repair, and function.