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Chapters
- 00:05Title
- 01:23Chemical Treatment of Zebrafish Embryos and Live Metabolic Profile Using the Seahorse XF Analyser
- 02:38Analysis of Samples and Oil Red O Staining
- 04:40Representative Metabolic Profile Analyses
- 05:00Conclusion
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
Tags
Metabolic Profile AnalysisZebrafish EmbryosGeneticsMitochondrial FunctionEtiologyDiseasesDiabetesObesityInstrumentationMonitoringParametersMitochondrial FunctionSeahorse Bioscience XF 24 Extracellular Flux AnalyzerIslet Capture MicroplatesBioenergeticsRespirationATP TurnoverProton LeakMaximum RespirationWild Type EmbryosGenetically Altered EmbryosPharmacological ManipulationMetabolic Disorders
