Inverse Transition Cycling

Inverse transition cycling is a protein purification method that exploits the reversible phase behavior of elastin-like polypeptides (ELPs) to separate recombinant fusion proteins from soluble cell lysates. ELPs remain soluble below their transition temperature but rapidly aggregate when heated above it; cooling resolubilizes them, enabling repeated cycles of heating, centrifugation, and recovery. This temperature-responsive process reduces reliance on chromatography and can concentrate target proteins through controlled phase transitions. In biology and biotechnology, inverse transition cycling supports scalable purification of proteins engineered with ELP tags and provides a practical platform for producing recombinant biomolecules.

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JoVE Core - Chemistry

Phase Transitions

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2020

Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to occupy...

Properties of Transition Metals

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2020

Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals. Figure 1: Periodic Table. The transition metals are located in groups 3–11 of the periodic table. The inner transition metals are in the two rows...

Cooperative Allosteric Transitions

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2020

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...

Liquid Phase Reactor: Sucrose Inversion

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2023

Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Both batch and continuous flow reactors are used in catalytic reactions. Packed beds, which use solid catalysts and a continuous flow, are the most common configuration. In the absence of an extensive recycle stream, such packed bed reactors are typically modeled as "plug flow". The other most common continuous reactor is a stirred tank, which is assumed to be perfectly...

Inverse Trigonometric Functions

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2025

Inverse trigonometric functions are fundamental mathematical tools that reverse the actions of standard trigonometric functions. While trigonometric functions map angles to ratios, inverse trigonometric functions perform the opposite operation by mapping a ratio back to its corresponding angle. These functions are essential in various applications, particularly in determining angles when given specific distances, such as calculating elevation angles in navigation and engineering.For a function...

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