Lesser Omentum

The lesser omentum is a double layer of peritoneum that connects the liver to the lesser curvature of the stomach and the first part of the duodenum, helping organize the upper abdominal organs. It consists mainly of the hepatogastric and hepatoduodenal ligaments; the latter encloses the portal triad, including the hepatic artery, portal vein, and bile duct, while the peritoneal layers provide routes for vessels, nerves, lymphatics, and connective tissue. This arrangement helps maintain organ relationships and creates a boundary around the lesser sac, making the lesser omentum important for understanding abdominal fluid movement, disease spread, and surgical access to the biliary tree and hepatic vessels.

Lesser Omentum - Related Videos

Research

JoVE EoE - Gynecologic Cancer

Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants

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2023

In this video, we establish an ex vivo omental organ culture overlaid with GFP-expressing ovarian cancer cells to study ovarian cancer colonization within the omental explant. This protocol analyzes immune cell structures within the omentum known as milky spots that promote metastases.

Mesothelial Cell Isolation: A Technique to Isolate Primary Mesothelial Cells From Human Omentum Tissue Specimen

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2023

This video describes the protocol to isolate primary human mesothelial cells from the human omentum specimen. The mesothelial cells can be used to establish an in vitro ovarian cancer model to investigate ovarian cancer cell adhesion, invasion, and proliferation.

A Method for Islet Transplantation to the Omentum in Mouse

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Cited by 4 •

2019

A method for the omental transplantation of islets in a mouse is introduced. The isolated islets are mixed with hydrogel and the mixture is placed into the omental pouch of a diabetic mouse. Then, the blood glucose is monitored, and immuno-histochemical analysis is performed.

An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum

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2024

This protocol describes the establishment of a three-dimensional (3D) ex vivo model of cancer cell-omentum interaction. The model provides a platform for elucidating pro-tumor mechanisms within the adipose niche and for testing novel therapies.

Intraoperative Strategy under Complex Vascular Adhesion for Laparoscopic Radical Resection of Bismuth-Corlette Type IIIb Perihilar Cholangiocarcinoma

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2026

The protocol presents a step-by-step laparoscopic protocol for radical resection of Bismuth-Corlette type IIIb perihilar cholangiocarcinoma. The procedure integrates preoperative PTCD, complex vascular dissection and repair, and biliary reconstruction for application in high-volume hepatobiliary centers.

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