Mammalian Tissue Culture
Mammalian Tissue Culture - Related Videos
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Cited by 28 •
2016
The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
07:52
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
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Cited by 10 •
2018
Here, we present a protocol to obtain 3D-culture systems in self-assembling peptide scaffolds to promote the differentiation of dedifferentiated human articular chondrocytes into cartilage-like tissue.
09:30
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
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Cited by 17 •
2015
Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.
02:45
Production and Collection of Pseudovirus Particles Using Mammalian Cell Culture
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2026
Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the procedure for infecting mammalian cells with green fluorescent protein (GFP)-encoding pseudovirus, monitoring viral replication and spread by fluorescence microscopy, and collecting virus-containing supernatant for downstream applications.
16:34
Laser Capture Microdissection of Mammalian Tissue
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Cited by 25 •
2007
Laser capture microscopy, also known as laser microdissection (LMD), enables the user to isolate small numbers of cells or tissues from frozen or ...
FAQs
The nutrient medium supplies several requirements at once, including energy, salts, amino acids, and growth factors. These components create the chemical environment needed to maintain cellular activity and support growth when the material is outside the body. Because requirements vary with the tissue and research goal, medium composition is an important factor influencing whether organized tissue or proliferating cell populations are maintained.
These conditions help preserve an environment in which mammalian cells and tissues can remain functional outside the body. Temperature, pH, and gas exchange are controlled together with the nutrient medium rather than treated as separate concerns. Careful regulation supports reliable biological responses and allows researchers to distinguish effects caused by an experiment from changes produced by unsuitable culture conditions.
The tissue source and the research objective strongly influence the form of the culture. Some approaches are intended to preserve organized tissue structure, whereas others generate cell populations that proliferate. This distinction affects the biological questions that can be addressed: organized cultures can retain aspects of tissue structure, while proliferating populations support studies requiring expandable cellular material.
A typical workflow begins by placing mammalian cells or a tissue fragment into a sterile vessel containing an appropriate nutrient medium. The culture is then maintained under controlled temperature, pH, and gas-exchange conditions, with contamination carefully limited. Researchers select the culture format according to whether they need preserved tissue organization or a proliferating cell population for subsequent investigation.
It is useful when researchers need to examine biological function under controlled conditions and manipulate experimental variables more precisely than in an intact organism. Cultured mammalian material can support investigations of disease mechanisms and responses to drugs. These experiments provide a model for examining how cells or tissues behave in relation to a defined research question.
Mammalian tissue culture provides a controllable platform for studying cell physiology, development, and the behavior of tissue-related systems. Its ability to maintain either organized tissue or proliferating cell populations makes it relevant to tissue engineering, where researchers investigate biological material outside the body. The approach also helps reduce reliance on whole-animal experiments while enabling precise experimental manipulation.