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Imaging Local Ca2+ Signals in Cultured Mammalian Cells
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Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

DOI:
10.3791/52516-v

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09:30 min

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March 03, 2015

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, , , ,
1Neurobiology and Behavior,University of California, Irvine, 2Physiology and Biophysics,University of California, Irvine

Chapters

  • 00:05Title
  • 02:07Loading Cells with Membrane-permeant Cal-520, ci-IP3 and EGTA
  • 03:46Ca2+ Image Acquisition
  • 05:08Automated Ca2+ Image Analysis
  • 07:26Results: IP3-evoked Local Ca2+ Signals in SH-SY5Y Cells Loaded with Cal-520 and ci-IP3
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.

Tags

Keywords: Ca2+ SignalingCytosolic CalciumCalcium MicrodomainsCalcium PuffsEMCCD CameraWide-field Epi-fluorescenceTIRF MicroscopyIP3-mediated Calcium SignalsAutomated AnalysisPython Algorithm

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