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Biology

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Imaging Local Ca2+ Signals in Cultured Mammalian Cells
 

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Article DOI: 10.3791/52516
March 3rd, 2015

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Summary March 3rd, 2015

Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.

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