Membrane Resealing
Membrane Resealing - Related Videos
04:34
An In Vitro Fluorescence-Based Assay to Measure Plasma Membrane Resealing Efficiency
0 Views •
2025
This video demonstrates an assay to study the plasma membrane resealing efficiency in mammalian cells. The cells are exposed to a bacterial pore-forming toxin, which forms pores in the cell membrane. In the presence of extracellular calcium ions, the pore causes calcium influx, inducing membrane-resealing events. The resealing efficiency is assessed using DNA-binding propidium iodide dye, which only enters cells without a resealed membrane.
10:07
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
0 Views •
Cited by 3 •
2019
Here we describe a high-throughput fluorescence-based assay that measures the plasma membrane resealing efficiency through fluorometric and imaging analyses in living cells. This assay can be used for screening drugs or target genes that regulate plasma membrane resealing in mammalian cells.
14:34
Surgical Protocol for a Large, Resealable Cranial Window Enabling Longitudinal, Multi-Modal Electrophysiology Recordings of Mouse Default Mode Network
0 Views •
2026
This protocol describes a two-phase surgical method to create a large, resealable, dura-sparing cranial window in mice. This technique enables chronic, multi-modal electrophysiological recordings from distributed, deep-brain networks, such as the Default Mode Network, over several weeks.
20:21
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
0 Views •
Cited by 47 •
2012
Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
0 Views •
Cited by 48 •
2010
Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.
FAQs
A rise in cytosolic Ca2+ acts as the signal that initiates repair after membrane rupture. When extracellular calcium enters through the damaged barrier, it triggers vesicle fusion, supplying membrane material to the injury site. This response links the extent of membrane disruption to activation of a repair mechanism that helps restore cellular integrity and support survival.
Vesicle fusion helps replace membrane at the rupture site, whereas endocytosis helps remove damaged membrane material from the cell surface. These processes therefore address complementary repair needs: one supplies replacement membrane, and the other clears injury-associated material. Considering both activities gives a fuller view of how cells rebuild and stabilize a disrupted barrier.
Cytoskeletal remodeling helps stabilize the area surrounding a membrane injury as repair proceeds. It works alongside vesicle fusion and endocytosis rather than replacing them, supporting organization of the repair site and removal or repositioning of damaged material. This coordination is important because restoring a barrier requires both membrane replacement and structural stabilization.
Mechanical injury, toxins, and physical stress can all disrupt cell membranes, making them relevant conditions for studying resealing. Examining repair responses across these types of damage can clarify how cells react to different causes of membrane failure. The resulting comparisons help connect the cellular repair process with broader questions about injury, cellular integrity, and recovery.
Studies can show how cells respond when membrane damage allows extracellular calcium to enter and threatens barrier function. Researchers can then relate calcium-triggered vesicle fusion, endocytosis, and cytoskeletal remodeling to cellular recovery or loss of integrity. This information helps explain why effective repair supports survival after membrane-disrupting events.
Membrane resealing provides a framework for examining how cells recover from membrane injury in conditions involving muscle damage or neurodegeneration. The process connects physical disruption with calcium entry, membrane replacement, and structural stabilization. Understanding these links can support research into cellular recovery and therapeutic strategies intended to improve how damaged cells withstand or respond to injury.