Microbial Oxidation

Microbial oxidation is the biological removal of electrons from organic or inorganic compounds by microorganisms, a process central to energy production and ecosystem chemistry. Enzymes catalyze oxidation reactions and transfer the released electrons through metabolic pathways to a terminal electron acceptor, such as oxygen or, under oxygen-limited conditions, nitrate or sulfate. These reactions allow microbes to conserve energy for growth while transforming carbon, nitrogen, sulfur, and other elements. In biology, microbial oxidation helps explain decomposition and biogeochemical cycles and supports applications including wastewater treatment, pollutant bioremediation, soil management, and the study of microbial energy metabolism.

Microbial Oxidation - Related Videos

Research

JoVE EoE - Bacterial Growth and Techniques

Measuring the Kinetic Isotope Effect on Microbial Electron Transport Using Deuterium Oxide

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2025

Source: Tokunou, Y., et al, Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (2018)This video demonstrates a whole-cell electrochemical method to assess how proton transfer affects extracellular electron transport (EET) in electroactive bacteria. By replacing protons with deuterons using deuterium oxide, the method measures changes in current to reveal a kinetic isotope effect, highlighting the role of...

Research

JoVE Journal - Environment
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A Microfluidic Platform to Investigate Microbial Precipitation of Metal Oxides in Porous Media

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2026

This protocol presents a microfluidic system for studying the microbial precipitation of manganese oxide minerals. Biomineral formation is measured in situ with optical microscopy and image analysis. This approach can be adapted to investigate the precipitation of other distinctively colored biogenic minerals in porous environments.

Education

JoVE Core - Chemistry

Oxidation Numbers

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2020

In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules. Oxidation Number (Oxidation State) In the case of an ionic compound, oxidation numbers are assigned based on the number of electrons transferred between reacting species. For example, in the formation of calcium...

Research

JoVE Journal - Biology
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Biology of Microbial Communities - Interview

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Cited by 3 •

2007

Pyruvate Oxidation

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2019

After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells. First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...

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