Microdialysis Catheter Placement
Microdialysis Catheter Placement - Related Videos
12:37
Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine
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Cited by 16 •
2016
Here we present a protocol to assess cardiopulmonary function in awake swine, at rest and during graded treadmill exercise. Chronic instrumentation allows for repeated hemodynamic measurements uninfluenced by cardiodepressive anesthetic agents.
06:57
Arterial Line Placement
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2023
Source: Sharon Bord, MD, Department of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA When monitoring patients, it is important to obtain values that are accurate and reliable. Blood pressure monitoring is one of the essential vital signs, and for a majority of patients, measuring it utilizing non-invasive techniques provides accurate values. However, there are situations in which the blood pressure requires more exact, specific, and reliable measurements.
07:47
The Supraclavicular Fossa Ultrasound View for Central Venous Catheter Placement and Catheter Change Over Guidewire
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Cited by 8 •
2014
The overall goal is to enable clinicians to insert a central venous catheter under real-time ultrasound guidance via the right supraclavicular fossa view of the lower part of the superior vena cava. This view is useful for different catheter insertion sites, thrombosis detection before insertion and ultrasound guided correction of misplaced catheters.
05:38
Custom-made Microdialysis Probe Design
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Cited by 9 •
2015
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
06:27
A Retrograde Implantation Approach for Peritoneal Dialysis Catheter Placement in Mice
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Cited by 1 •
2022
This article describes modifications of a procedure to implant a peritoneal dialysis catheter in a murine model to avoid major technical issues observed with the conventional techniques.
FAQs
The membrane permits molecules in the surrounding extracellular space to diffuse into the perfusion fluid according to concentration gradients. Consequently, the dialysate can contain locally available neurotransmitters, metabolites, and drugs without requiring researchers to remove the surrounding tissue. Membrane-based exchange connects local chemistry with later analytical measurements while supporting preservation of local physiology.
The catheter must be perfused with fluid so molecules can cross the membrane and enter the collected dialysate. Controlled perfusion is essential because the resulting sample must represent local extracellular chemistry consistently. It also supports interpretation of tissue responses, making perfusion control a central condition for reliable, localized measurements rather than a minor operational detail.
Accurate positioning determines which tissue region contributes molecules to the dialysate. A misplaced catheter may therefore fail to represent the biological location under investigation, whereas correct placement supports localized, time-resolved sampling. In brain studies and work involving other tissues, the position must be considered when relating measured neurotransmitters, metabolites, or drugs to local physiology.
The workflow begins by positioning the catheter in the tissue region of interest, followed by perfusing its semipermeable membrane with fluid. Researchers then collect the resulting dialysate and analyze its chemical contents. Maintaining accurate placement and controlled perfusion throughout these stages helps produce samples that support meaningful interpretation of extracellular conditions and tissue responses.
Researchers can apply the approach to studies of neural signaling, metabolism, pharmacology, and disease-related changes. In the brain or other tissues, localized sampling reveals extracellular neurotransmitters, metabolites, and drugs over time. This combination of chemical information and spatial targeting helps connect molecular changes with biological processes occurring in a defined tissue environment.
A properly positioned catheter can provide localized, time-resolved information about extracellular chemicals in living tissue. Analysis of the dialysate may reveal changes in neurotransmitters, metabolites, or drugs, while the sampling location helps relate those changes to local physiology. These results are most useful when researchers account for placement accuracy and controlled perfusion during interpretation.