Mitochondrial Rna Editing
Mitochondrial Rna Editing - Related Videos
02:23
RNA Editing
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2020
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
09:19
RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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Cited by 1 •
2014
A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as therapeutics.
08:50
A Nonsequencing Approach for the Rapid Detection of RNA Editing
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2022
Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
FAQs
Guide RNAs provide sequence information through base pairing with mitochondrial transcripts. This pairing identifies regions that do not match the required mature message and directs the editing complex to those sites. The resulting alignment helps specify where uridines must be inserted or removed, making guide RNA recognition central to editing accuracy and transcript maturation.
The editing cycle coordinates transcript cutting, nucleotide modification, and fragment rejoining. Cleavage exposes the targeted region, uridine addition or removal corrects the local sequence, and ligation reconnects the RNA. Repeating these steps can progressively convert an initially incomplete or mismatched transcript into a continuous message capable of restoring an open reading frame.
The process can modify mitochondrial messenger RNAs, ribosomal RNAs, and transfer RNAs. Editing therefore affects more than the coding sequence of a single message: it can contribute to the maturation of several RNA classes required for mitochondrial gene expression. In kinetoplastid mitochondria, these changes are linked to production of components needed for oxidative phosphorylation.
A mechanistic analysis would follow guide RNA pairing, editing-complex action, cleavage, uridine insertion or deletion, and ligation. Researchers can then consider whether the resulting transcript has a restored open reading frame or a mature RNA sequence. This sequence-based view connects individual molecular steps with the final state of mitochondrial RNA.
Such transcripts demonstrate that transcription alone does not always produce the final functional message in the organisms described. Subsequent editing supplies or removes uridines so the RNA can acquire the sequence needed for proper interpretation. Examining this dependence reveals how mitochondrial gene expression can be distributed across transcription and post-transcriptional processing.
Editing can generate mature mitochondrial RNAs needed for gene expression associated with oxidative phosphorylation. Studying the process therefore links RNA sequence correction to the production of mitochondrial messages and other functional RNA classes. This connection helps biology researchers investigate how organelle RNA processing supports mitochondrial activity without treating transcription as the only relevant stage.
Research in this area illuminates organelle biology, RNA-processing mechanisms, and evolutionary innovation. The unusual use of guide-directed uridine insertion or deletion provides a system for examining how genetic information can be processed after transcription. It also offers context for investigating mitochondrial dysfunction and related diseases when RNA maturation is relevant to organelle function.