Mouse Peritoneal Injection
Mouse Peritoneal Injection - Related Videos
04:32
Isolation of Mouse Peritoneal Cavity Cells
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Cited by 445 •
2010
The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.
03:20
Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model
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2023
In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent...
03:14
Assessing the Survival of a Bacterial Pathogen in Mouse Peritoneal Macrophages
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2026
Source: Zhang, C. et al. Development and Assessment of Intracellular Infection Models for Staphylococcus aureus. J. Vis. Exp. (2025)This video demonstrates the quantification of intracellular survival of a methicillin-resistant bacterial pathogen in mouse peritoneal macrophages. It outlines the steps involved in infection, extracellular bacterial clearance, host cell lysis, and colony enumeration to assess bacterial survival within host macrophages.
04:20
Intra-uterine Cancer Cell Injection: A Technique to Deliver Cancer Cells for Establishing Intra-peritoneal Ovarian Cancer Mouse Model
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2023
In this video, we demonstrate intra-uterine injection of ovarian cancer cells in a mouse model. This orthotopic murine model helps us to non-invasively monitor tumor growth and evaluate the efficacy of new therapies for the prevention and treatment of recurrent ovarian cancer.
03:41
Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models
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2023
In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.
FAQs
After administration, the experimental material can distribute within the peritoneal cavity and cross the peritoneal lining. This creates two broad experimental outcomes: effects that remain associated with the abdominal cavity and effects that become systemic after absorption. The intended outcome depends on the material and study design, making this route useful for examining both localized responses and broader biological changes.
The peritoneal lining functions as the interface through which administered substances can move beyond the cavity. Its role connects placement of the material with subsequent absorption and biological response. For experimental interpretation, researchers must distinguish the initial distribution of a substance inside the cavity from the later effects that may appear throughout the mouse.
Reliable results depend on controlled administration and careful handling of the animal. Inconsistent restraint, needle placement, tissue injury, or unintended organ puncture can alter the delivered exposure or produce biological effects unrelated to the experimental material. Standardizing technique therefore helps reduce variability and makes treatment, absorption, toxicity, or disease-model findings easier to compare.
The route can accommodate fluids, drugs, cells, and other experimental materials, allowing investigators to match the administered substance to the biological question. A drug may support treatment or pharmacology studies, cells may be relevant to immunology or disease models, and other materials may be used to examine biological responses or toxicological effects under controlled conditions.
The procedure begins with appropriate restraint of the mouse, followed by insertion of a needle through the abdominal wall into the peritoneal cavity. The experimental material is then delivered into that space. Careful execution throughout these stages is important because inaccurate placement can cause tissue injury, unintended organ puncture, or variation in the exposure received by the animal.
Researchers apply this route in pharmacology, immunology, toxicology, and disease-modeling studies. It can support controlled investigations of how an administered material is absorbed, how treatment changes biological responses, or how exposure contributes to a modeled disease process. The technique is especially relevant when investigators need a defined abdominal delivery route while monitoring local or systemic outcomes.
Studies using this approach can evaluate absorption, treatment effects, toxicity, and biological responses after administration. Depending on the experimental material and design, investigators may examine localized effects within the peritoneal cavity, systemic consequences after crossing the lining, or changes associated with a disease model. Careful technique strengthens interpretation by limiting procedure-related effects.