Ms5 Stromal Cells
Ms5 Stromal Cells - Related Videos
03:58
Generating Schwann Cell-Like Cells from Bone Marrow Stromal Cells
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2025
This video demonstrates a method for obtaining Schwann cell-like cells from multipotent bone marrow stromal cells (BMSCs). Initially, the BMSCs undergo hypoxia treatment, followed by directed differentiation into neuronal progenitor cells. Subsequently, specific media and culture conditions are employed to induce the transformation of these progenitor cells into Schwann cell-like cells.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
08:37
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
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Cited by 86 •
2017
This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
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2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
07:06
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Cited by 171 •
2017
Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
FAQs
Support arises through two complementary routes: direct contact with the adherent stromal layer and soluble signals released by the stromal cells. Considering both routes is important because a co-culture can reproduce interactions that are not captured by studying hematopoietic cells alone. This combined support helps maintain blood-forming cells while also providing a context for examining lineage differentiation.
The adherent layer provides a stable cellular context in which hematopoietic cells interact with stromal cells rather than existing in isolation. It therefore serves as an in vitro representation of key features of the bone marrow microenvironment. Researchers can use this arrangement to examine how cellular surroundings influence blood-cell development under more controlled conditions than direct study of the whole organism.
These co-cultures support investigations of hematopoietic stem and progenitor cell maintenance, lineage differentiation, and communication between stromal and blood-forming cells. Studying these processes together helps connect the persistence of early hematopoietic populations with the production of differentiated blood cells. The system is therefore useful for analyzing how the microenvironment contributes to multiple stages of hematopoiesis.
A controllable co-culture provides a setting for introducing or examining changes that may affect blood-cell production, then assessing their influence on hematopoietic maintenance or differentiation. The stromal and hematopoietic components can be considered as interacting parts of the model, helping researchers distinguish effects associated with the cellular microenvironment from outcomes observed in blood-forming cells.
The general workflow is to establish the Ms5 cells as an adherent stromal layer and then culture hematopoietic cells in association with that layer. Researchers subsequently examine maintenance, differentiation, or interactions between the two cell populations. Because the model is performed in vitro, experimental conditions can be controlled while the resulting changes in blood-cell development are evaluated.
They are especially useful when a study requires a controllable model of the cellular environment supporting blood-cell development. Applications include investigating hematopoietic stem and progenitor cell behavior, studying stromal interactions, and evaluating how genetic, molecular, or pharmacological perturbations influence blood-cell production. The system also provides a practical platform for connecting cellular mechanisms with broader questions in hematopoiesis.