Observation Channel
Observation Channel - Related Videos
04:51
Observational Research
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2023
Source: Laboratories of Gary Lewandowski, Dave Strohmetz, and Natalie Ciarocco—Monmouth University If you want to know how someone thinks or feels, you can ask that person questions. Another approach is to observe how the person is acting or look for indicators of how they acted in the past. While observations may seem revealing, it isn’t always easy to know if they are truly accurate. For instance, you may see a person smiling and assume they are happy, when in reality they are annoyed and...
01:19
Ion Channels
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2019
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange. Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
02:30
Naturalistic Observations
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2020
If you want to understand how behavior occurs, one of the best ways to gain information is to simply observe the behavior in its natural context. However, people might change their behavior in unexpected ways if they know they are being observed. How do researchers obtain accurate information when people tend to hide their natural behavior? As an example, imagine that your professor asks everyone in your class to raise their hand if they always wash their hands after using the restroom. Chances...
05:02
Observation and Inspection
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2023
Source: Jaideep S. Talwalkar, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT Observation and inspection is fundamental to physical examination and begins at the first point of contact with a patient. While observation and inspection are often used interchangeably, observation is a general term that refers to the careful use of one's senses to gain information. Inspection is an act limited to what one can observe visually, and when referring to physical examination,...
16:00
Fabrication of the Thermoplastic Microfluidic Channels
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2008
Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase columns.
FAQs
The selected illumination or excitation condition determines which signal from a biological sample can be generated for detection. Because different targets or events may respond to different conditions, changing excitation can alter which structures become visible in a channel. Matching this condition with the intended signal helps researchers distinguish biological features and compare them across imaging measurements.
Wavelength-specific filters help separate the desired emitted or reflected light from other optical signals. Together with the other optical components and detector, they direct a particular signal into the selected channel. This separation allows researchers to display labeled targets individually, reducing confusion between signals and supporting clearer comparisons within the same biological specimen.
Acquiring multiple channels separately and overlaying them allows researchers to compare the locations of different labeled targets in one specimen. When signals appear together, the comparison can support colocalization analysis, meaning an assessment of whether features occupy corresponding locations. This approach helps investigate biological organization and possible relationships between structures or molecules.
A typical workflow selects the illumination or excitation condition, configures wavelength-specific filters and related optical components, and directs the resulting emitted or reflected light to a detector. The detector records the signal for display and analysis. If several targets are being examined, researchers can acquire their channels separately before overlaying the resulting images.
A defined channel can isolate the signal associated with a selected labeled target or cellular event while the specimen is observed over time. In live-cell studies, this supports examination of cellular dynamics rather than only a static image. Using separate channels can also help compare changing signals from multiple targets within the same living sample.
Channel-based imaging supports studies of biological organization, interactions, and dynamics. Researchers can use the recorded signals to distinguish structures or molecules, compare their distributions through overlays, and perform colocalization analysis. The same imaging strategy also contributes to quantitative studies, where channel-specific signals provide distinct measurements for examining biological patterns within a specimen.