Lineage Tracking
Lineage Tracking - Related Videos
10:55
Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
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Cited by 12 •
2017
A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.
03:54
Live Imaging and Single-Cell Tracking to Monitor Neural Lineage in Adult Neural Stem Cells
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2025
Source: Gómez-Villafuertes, R., et. al., Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations. J. Vis. Exp. (2017) The video demonstrates the process of preparing, imaging, and tracking adult neural stem cells in real time to study cellular behaviors, including division and differentiation.
01:21
Lineage Commitment
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2023
Commitment is the process whereby stem cells: lose their ability to form all cell types and irreversibly change into a specific type. The multipotent hematopoietic stem cells, (HSCs), differentiate into the multipotent hematopoietic progenitor cells, (HPCs). The HPCs express many lineage-specific cytokine receptors. Each of these receptors binds specific cytokines, activates distinct signaling pathways, and expresses a particular gene set. The HPCs further differentiate to form committed...
10:41
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
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Cited by 9 •
2015
Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.
07:48
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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Cited by 15 •
2015
In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.
FAQs
A heritable marker links a labeled progenitor to its descendants because daughter cells retain the fluorescent reporter or genetic barcode. Detecting that signal later allows investigators to reconstruct which cells share ancestry and how a progenitor contributes to a tissue. This makes marker inheritance central to following lineage relationships over time.
Fluorescent reporters provide a visible signal that researchers can follow through imaging, while genetic barcodes are identified through sequencing or targeted molecular assays. Both approaches preserve information about ancestry, but they use different detection strategies. Selecting between them determines whether lineage relationships are observed visually or recovered from molecular measurements.
The resulting lineage information can show how stem and progenitor cells generate diverse cell lineages, how individual cells contribute to tissues, and how clones expand. These patterns connect ancestry with biological outcomes, allowing researchers to examine not only which cells are related, but also how their descendants shape development or population change.
Researchers first label a progenitor with a heritable fluorescent reporter or genetic barcode. They then follow or collect descendant cells and detect the inherited marker using imaging, sequencing, or a targeted molecular assay. Comparing marker patterns among cells reveals shared ancestry and supports reconstruction of the lineage relationships being studied.
In developmental biology, lineage tracking supports fate mapping and helps determine how progenitor populations generate different descendants. Following markers through time can connect an early labeled cell with later tissue contributions and reveal the organization of developing lineages. This makes the approach useful for examining cell fate and tissue formation.
Disease studies can use lineage information to examine clonal expansion in cancer and to follow population change during evolution. In regenerative medicine, the same approach helps investigate how stem and progenitor cells contribute to tissues. These applications connect cell ancestry with abnormal growth, tissue renewal, and the behavior of repair-related cell populations.