Organic Extraction
Organic Extraction - Related Videos
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
06:30
Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method
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2026
This protocol describes a rapid, reproducible organic solvent-based extraction and precipitation method for purifying elastin-like polypeptide (ELP) from Escherichia coli, providing a potentially scalable alternative to conventional ELP purification methods.
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Extraction - Concepts
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2020
Extraction Extraction is a common technique used in organic chemistry to isolate a target compound. In the extraction process, a solute is transferred from one phase to another to separate it from unreacted starting materials or impurities. Extraction is also used to facilitate the isolation of a solute from a reaction solvent that is difficult to remove by evaporation, such as a solvent with a high boiling point. Generally, there are three types of extractions. First, in solid-liquid...
19:00
Extraction - Student Protocol
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2020
Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Cellulose RecoveryExpand Extraction and filtration can separate compounds based on their solubility properties. In this lab, you'll separate a mixture of cellulose, caffeine, and benzoic acid based on their solubilities in dichloromethane, or DCM, and water. Caffeine and benzoic acid are both soluble in DCM, but cellulose is not. Thus, in this part, you'll first dissolve as much of the mixture as...
06:42
Extraction of Biomarkers from Sediments - Accelerated Solvent Extraction
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The distribution of a group of organic biomarkers called glycerol-dialkyl glycerol-tetraethers (GDGTs), produced by a suite of archaea and bacteria, were found in modern sediments to change in a predictable manner in response to air or water temperature1,2. Therefore, the distribution of these biomarkers in a sequence of sediments of known age can be used to reconstruct the evolution of air and/or water temperature on...
FAQs
Polarity determines whether a molecule preferentially dissolves in the organic or aqueous phase, while the partition coefficient describes how that molecule distributes between the two phases. Compounds with greater affinity for one solvent accumulate there more strongly. These properties influence recovery, fraction purity, and the suitability of the resulting sample for biochemical or analytical measurements.
Immiscibility allows the sample to form two distinct liquid phases rather than one uniform solution. Target molecules can distribute between these phases according to their chemical properties, after which the phases can be separated physically. Clear phase separation is therefore essential for collecting an enriched fraction and reducing unwanted components from the biological sample.
Concentration affects the amount of target compound available to partition between the two solvents and can influence the composition of each recovered fraction. Along with polarity and partition coefficient, it helps determine where molecules accumulate. Controlling or interpreting concentration is important when comparing extracts, assessing cellular composition, or preparing samples for quantitative analysis.
Drying or solvent removal prepares the isolated fraction for subsequent analysis and can reduce interference from the extraction liquid. This post-separation step concentrates or stabilizes the recovered material in a form compatible with methods such as chromatography, spectroscopy, or biochemical assays. Its use depends on the intended measurement and the properties of the extracted compounds.
A typical workflow begins with a biological sample, introduces an organic solvent, and allows compounds to distribute between the organic and aqueous phases. The phases are then separated, and the collected fraction may undergo drying or solvent removal. The resulting preparation can be directed toward biochemical assays, chromatography, spectroscopy, or other analyses of its molecular contents.
In biology, the method can be applied to cells, tissues, and biological fluids. It may recover lipids, pigments, metabolites, and other biomolecules, depending on their solubility and distribution between the selected phases. This range makes extraction useful for examining cellular composition and for preparing chemically distinct fractions before further characterization.
Separated fractions provide preparations enriched in particular classes of biological molecules, which can improve the relevance of downstream measurements. Researchers can use them in biochemical assays to examine activity, chromatography to separate components further, or spectroscopy to characterize molecular features. These analyses help connect chemical composition with cellular function and biological sample properties.