Oxygen Scavenging Solution

An oxygen scavenging solution is a chemical preparation that removes dissolved or entrapped oxygen from a biological environment, helping create controlled low-oxygen or oxygen-free conditions. Its active components react with molecular oxygen through reduction or oxidation processes, decreasing oxygen availability and limiting reactions driven by oxidative chemistry. In biology, these solutions support anaerobic culture, protect oxygen-sensitive enzymes and biomolecules, and help maintain defined redox conditions during experiments. By controlling oxygen exposure, they improve the stability, reproducibility, and interpretation of studies involving microbial physiology, cellular metabolism, protein function, and other oxygen-dependent biological processes.

Oxygen Scavenging Solution - Related Videos

Research

JoVE Journal - Biochemistry

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

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Cited by 3 •

2019

Protocatechuate 3,4-dioxygenase (PCD) can enzymatically remove free diatomic oxygen from an aqueous system using its substrate protocatechuic acid (PCA). This protocol describes the expression, purification, and activity analysis of this oxygen scavenging enzyme.

Research

JoVE Journal - Bioengineering
Free Sample

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke

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Cited by 18 •

2012

Spin-trapping ESR spectroscopy was used to study the effect of plant antioxidants lycopene, pycnogenol and grape seed extract on scavenging gas-phase free radicals in cigarette smoke.

Catalytic Scavenging of Plant Reactive Oxygen Species In Vivo by Anionic Cerium Oxide Nanoparticles

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Cited by 24 •

2018

Here, we present a protocol for the synthesis and characterization of cerium oxide nanoparticles (nanoceria) for ROS (reactive oxygen species) scavenging in vivo, nanoceria imaging in plant tissues by confocal microscopy, and in vivo monitoring of nanoceria ROS scavenging by confocal microscopy.

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

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Cited by 27 •

2011

We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.

Education

JoVE Science Education - Advanced Biology

Detecting Reactive Oxygen Species

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2023

Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...

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