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Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California
Chapters
- 00:05Title
- 00:11Introduction
- 00:56Wide Field of View Imaging of Partial Pressure of Oxygen in Cortical Vasculature of a Rat
- 04:55High Resolution Two-photon pO2 Imaging in Cortical Micro Vasculature of a Mouse
- 08:22Conclusion
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
Tags
Cerebral Blood Oxygenation MeasurementOxygen-dependent Quenching Of PhosphorescenceSpatiotemporal CharacteristicsNeuro-metabolic-vascular RelationshipPO2 Measurement ModalitiesLarger Fields Of ViewHigher Spatial ResolutionHigher Temporal ResolutionNormal Brain FunctioningNeurovascular DiseasesStrokeAlzheimer’s DiseaseHead InjuryOptical Imaging ModalitiesHemoglobin AbsorptionVisible And Near Infrared RangeMultispectral MeasurementBlood Oxygenation EstimationPartial Pressure Of Oxygen (pO2)Oxygen-sensitive Dyes
