Ozone Layer

The ozone layer is a region of Earth’s stratosphere containing elevated concentrations of ozone, a form of oxygen that protects life by absorbing harmful ultraviolet radiation. High-energy ultraviolet light splits oxygen molecules into individual oxygen atoms, which then combine with O2 to form O3; ozone also absorbs ultraviolet energy and breaks down, creating a dynamic cycle. In biology, this natural shield limits exposure to ultraviolet-B and ultraviolet-C radiation that can damage DNA, impair photosynthesis, and harm organisms. Ozone depletion, driven largely by chlorine- and bromine-containing compounds, increases ultraviolet exposure and affects human health, ecosystems, and agricultural productivity.

Ozone Layer - Related Videos

Education

JoVE Science Education - Environmental Sciences

Measuring Tropospheric Ozone

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2023

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Ozone is a form of elemental oxygen (O3), a molecule of three oxygen atoms bonded in a structure that is highly reactive as an oxidizing agent. Ozone occurs in both the stratosphere and the troposphere levels of the atmosphere. When in the stratosphere (located approximately 10-50 km from the earth’s surface), ozone molecules form to the ozone layer and help prevent harmful UV rays from reaching Earth’s surface. In...

Research

JoVE Journal - Medicine

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

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Cited by 7 •

2017

This study describes the successful generation of a new chronic obstructive pulmonary disease (COPD) animal model by repeatedly exposing mice to high concentrations of ozone.

Thin-Layer Chromatography - Concepts

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2020

Chromatography Chromatography is a technique used in organic chemistry to separate compounds in a mixture based on their differences in solubility between two different phases. The concept is similar to liquid-liquid extraction, except in chromatography, the two phases consist of the stationary phase and the mobile phase. The stationary phase is a solid — typically a microscale hydrogel bead — while the mobile phase is a carrier solvent. In traditional chromatography, the stationary phase is...

Thin-Layer Chromatography - Student Protocol

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2020

Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Effects of Mobile Phase Composition on Compound SeparationExpand In this section of the lab, you will use TLC to compare how well pure hexane and a mixture of 60% hexane and 40% ethyl acetate can separate phenol, benzoic acid, and butyl phenyl ether by measuring the distance the compound traveled in each solvent. Before starting the lab, put on a lab coat, safety glasses, and nitrile gloves.

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

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Cited by 7 •

2015

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

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