Perfusion Chamber Imaging
Perfusion Chamber Imaging - Related Videos
27:58
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
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Cited by 8 •
2007
We demonstrate fabrication of a simple microfluidic device that can be integrated with standard electrophysiology setups to expose microscale surfaces of a brain slice in a well controlled manner to different neurotransmitters.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
07:59
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
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Cited by 25 •
2017
Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.
10:38
Preparing Individual Drosophila Egg Chambers for Live Imaging
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Cited by 19 •
2012
The Drosophila egg chamber is an excellent model for studying the mechanisms of mRNA localization. In order to capture the dynamic events that underpin the processes of localization, rapid high resolution imaging of live tissue is required. Here, we present a protocol for dissection and imaging of live samples with minimal disruption.
09:51
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
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Cited by 5 •
2018
This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.
FAQs
Controlled fluid flow exposes cells to defined shear stress, the physical force exerted by moving medium. This mechanical input can alter cellular behavior and allows imaging to connect visible responses with a specified flow condition. The approach is therefore useful for examining how cells respond dynamically to their surrounding environment rather than only to chemical changes.
Continuous medium replacement helps maintain a more consistent cellular environment during imaging. Nutrients and gases are delivered while waste is removed, reducing changes that might arise during prolonged observation. This supports experiments in which researchers need to distinguish responses to an added compound or fluid-flow condition from effects caused by declining culture conditions.
The system can regulate medium delivery through pumping or pressure-driven flow and can maintain conditions such as temperature and pH. It also controls exposure to nutrients, gases, experimental compounds, and defined fluid movement. Managing these variables gives researchers a way to study cellular responses under specified environmental and mechanical conditions.
A biological sample is placed in a specialized chamber connected to a medium-delivery system, and the chamber is positioned for live-cell microscopy. Researchers then establish the desired flow and maintain relevant conditions, including temperature and pH, while supplying medium or experimental compounds. Imaging proceeds as the system continuously delivers inputs and removes waste.
Perfusion chamber imaging is useful when experiments require observation of living samples during changing or sustained conditions. Applications include studying cell signaling, migration, adhesion, barrier function, and responses to drugs or other experimental compounds. Continuous observation helps reveal dynamic behavior that could be missed when samples undergo repeated handling or medium exchange.
The method provides real-time visual information about how biological samples respond to chemical exposure, fluid flow, or changing culture conditions. Researchers can observe processes such as migration, adhesion, signaling-related behavior, and barrier responses while the experiment continues. Because handling is reduced, the recorded response is less disrupted by repeated manual medium changes.