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JoVE Journal
Developmental Biology

This content is Open Access.

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
 

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Article DOI: 10.3791/55331
May 17th, 2017

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Summary May 17th, 2017

Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.

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