Pollen Source Control
Pollen Source Control - Related Videos
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
08:11
Collection and Identification of Pollen from Honey Bee Colonies
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Cited by 8 •
2021
We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.
10:13
Working with Hot and Cold Sources
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2023
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Misinformation & Source Credibility
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The rapid expansion of online information has increased access to knowledge while also increasing the risk of misinformation. Sources that appear authoritative may lack scholarly rigor, which makes credibility assessment a critical part of research literacy. Evaluating the reliability of information helps prevent the inclusion of weak, biased, or misleading material in academic work. Publication Source and Authorship A primary consideration is the publication source. Scholarly journals and...
07:54
Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
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Cited by 6 •
2020
This protocol describes tubulin purification from small/medium-scale sources such as cultured cells or single mouse brains, using polymerization and depolymerization cycles. The purified tubulin is enriched in specific isotypes or has specific posttranslational modifications and can be used in in vitro reconstitution assays to study microtubule dynamics and interactions.
FAQs
Maintaining a known male parent lets researchers connect the resulting fertilization event to a specific source. This improves confidence when examining inheritance, reproductive compatibility, or traits in breeding material. Without source control, unwanted pollen can introduce an unplanned male parent, making genetic studies, hybrid production, and interpretation of offspring outcomes less reliable.
These approaches provide different ways to reduce the chance that unintended pollen reaches a flower. Floral isolation and bagging restrict opportunities for outside pollen movement, while emasculation and male-sterile plants prevent the flower or plant from contributing unwanted pollen. Selecting among these measures helps preserve the intended parentage of a cross.
Selected pollen must be transferred when the stigma is receptive for the planned fertilization to proceed. This condition links the timing of pollen delivery with the female reproductive surface rather than treating pollen placement as sufficient by itself. Accounting for receptivity therefore helps researchers perform crosses consistently and interpret unsuccessful or successful fertilization more accurately.
A typical sequence begins by identifying the intended male and female plants, reducing opportunities for unwanted pollination, and applying a protective or reproductive control measure. Researchers then transfer the selected pollen to a receptive stigma while maintaining the planned cross. These steps preserve a clear connection between pollen source and fertilization outcome.
The approach is useful whenever the identity of the male parent affects the question being studied. Applications include genetic studies, crop improvement, hybrid seed production, and investigations of pollination, inheritance, or reproductive compatibility. In each case, controlling pollen movement helps distinguish planned biological relationships from effects caused by accidental cross-contamination.
By reducing unintended pollen, the method helps breeders maintain the selected parental combination used to produce experimental or improved material. This is particularly relevant when researchers need offspring associated with a planned cross rather than an unknown pollen source. The resulting control supports more accurate evaluation of inherited characteristics and hybrid outcomes.