Polysaccharide Layer

A polysaccharide layer is a carbohydrate-rich coating or matrix that surrounds a cell or forms part of its extracellular environment, influencing surface interactions and protection. Enzymes assemble sugar monomers through glycosidic bonds into branched or linear polymers that bind water and organize with proteins, lipids, or other extracellular components to form capsules, slime layers, and glycocalyx. In biology, polysaccharide layers help cells adhere to surfaces, resist dehydration, and limit access by harmful agents, while their composition supports research on microbial communities, host-pathogen interactions, biofilms, and potential antimicrobial strategies.

Polysaccharide Layer - Related Videos

Education

JoVE Core - Microbiology

Biosynthesis of Polysaccharides

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2025

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Cellulose and Pectic Polysaccharides

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2023

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds. As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...

Research

JoVE Journal - Chemistry

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

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2025

This study compared the polysaccharide contents of Hippophae rhamnoides subsp. sinensis Rousi and Hippophae gyantsensis (Rousi) Y. S. Lian obtained through hot water extraction and quantified through phenol-sulfuric acid colorimetry. We also investigated in vitro antioxidant activities of polysaccharides from these two Hippophae species through 2,2-diphenyl-1-picrylhydrazyl free radical scavenging experiments.

Thin-Layer Chromatography - Concepts

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2020

Chromatography Chromatography is a technique used in organic chemistry to separate compounds in a mixture based on their differences in solubility between two different phases. The concept is similar to liquid-liquid extraction, except in chromatography, the two phases consist of the stationary phase and the mobile phase. The stationary phase is a solid — typically a microscale hydrogel bead — while the mobile phase is a carrier solvent. In traditional chromatography, the stationary phase is...

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

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Cited by 7 •

2015

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

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