Post Capture Pcr
Post Capture Pcr - Related Videos
03:41
Agarose Gel Electrophoresis of DNA Amplicons Post PCR: A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success
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2025
In this video, we demonstrate the separation of bacterial PCR-amplified DNA using agarose gel electrophoresis. Agarose gel functions as a molecular sieve, enabling the negatively-charged DNA to migrate based upon their size under an applied electric field.
05:03
Laser Capture Microdissection to Isolate Purkinje Cells from a Human Post-Mortem Cerebellum Tissue
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2025
This video demonstrates how to isolate Purkinje cells from a human post-mortem cerebellar tissue using laser capture microdissection.
12:29
mRNA Interactome Capture from Plant Protoplasts
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Cited by 1 •
2017
Here, we present an interactome capture protocol applied to Arabidopsis thaliana leaf mesophyll protoplasts. This method critically relies on in vivo UV crosslinking and allows for the isolation and identification of plant mRNA-binding proteins from a physiological environment.
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Cited by 9 •
2016
Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.
13:27
PCR: The Polymerase Chain Reaction
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2023
The polymerase chain reaction, or PCR, is a technique used to amplify DNA through thermocycling – cyles of temperature changes at fixed time intervals. Using a thermostable DNA polymerase, PCR can create numerous copies of DNA from DNA building blocks called dNTPs. There are three steps in PCR: denaturation, annealing, and elongation. Denaturation is the first step in the cycle and causes the DNA to melt by disrupting hydrogen bonds between the bases resulting in single-stranded DNA. Annealing...
FAQs
Each cycle separates the DNA or complementary DNA strands by denaturation, allows primers to bind selected sequences during annealing, and uses a polymerase to extend those primers. Repeating these steps increases the amount of the targeted product from the captured sample. This sequence-specific cycling makes previously limited material suitable for downstream molecular analysis.
Capture alone isolates the cells, tissue regions, or nucleic acid molecules of interest, but the material must then be lysed so its nucleic acids become available for analysis. The resulting DNA or complementary DNA provides templates for amplification. This connection between physical isolation and molecular copying preserves information from a defined biological source rather than from a mixed sample.
Primer selection determines which target sequences are copied during the reaction. Primers anneal to complementary regions in the available DNA or complementary DNA, directing polymerase extension toward the selected products. Consequently, the assay can focus on gene expression targets, genotype-associated sequences, or mutation-related regions, depending on the biological question and the captured material.
A typical workflow begins by isolating selected cells, tissue regions, or nucleic acid molecules. The captured material is then lysed, and its DNA or complementary DNA is subjected to repeated denaturation, primer annealing, and polymerase extension. The amplified products can subsequently be examined to evaluate selected molecular targets from the isolated biological material.
Researchers can choose this approach when molecular information from a defined cell or microscopic tissue region might be obscured in a bulk sample. Linking capture with amplification allows analysis of limited biological material while retaining its source context. This is especially relevant when biological differences among individually captured cells or localized tissue areas are central to the study.
The amplified products can support gene expression profiling, genotyping, mutation detection, and validation of selected targets. In each case, the analysis connects a molecular signal to material obtained from a particular captured cell or tissue region. That linkage helps investigators examine molecular variation that may not be distinguishable when the same type of material is analyzed collectively.