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JoVE Journal
Biochemistry

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mRNA Interactome Capture from Plant Protoplasts
 

mRNA Interactome Capture from Plant Protoplasts

Article DOI: 10.3791/56011
July 28th, 2017

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Summary July 28th, 2017

Here, we present an interactome capture protocol applied to Arabidopsis thaliana leaf mesophyll protoplasts. This method critically relies on in vivo UV crosslinking and allows for the isolation and identification of plant mRNA-binding proteins from a physiological environment.

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