Pressure Injector
Pressure Injector - Related Videos
13:36
Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
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Cited by 10 •
2010
Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
07:26
Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Cited by 5 •
2013
The laser heated diamond anvil cell combined with synchrotron micro-diffraction techniques allows researchers to explore the nature and properties of new phases of matter at extreme pressure and temperature (PT) conditions. Heterogeneous samples can be characterized in situ under high pressure by 2D mapping and combined powder, single-crystal and multigrain diffraction approaches.
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
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Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
02:34
Vapor Pressure
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2020
When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase molecules move randomly about, they will occasionally collide with the surface of the condensed phase, and in some cases, these collisions will result in the molecules re-entering the condensed phase. The change from the gas phase to the liquid is called condensation. When the rate of condensation becomes equal to the rate of vaporization, neither the amount of the liquid nor the amount of the vapor...
03:28
Vapor Pressure Lowering
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2020
The equilibrium vapor pressure of a liquid is the pressure exerted by its gaseous phase when vaporization and condensation are occurring at equal rates: Dissolving a nonvolatile substance in volatile liquid results in a lowering of the liquid’s vapor pressure. This phenomenon can be explained by considering the effect of added solute molecules on the liquid's vaporization and condensation processes. To vaporize, solvent molecules must be present at the surface of the solution. The presence of...
FAQs
The expelled volume depends on three linked settings: applied pressure, pulse duration, and micropipette geometry. Changing any of these parameters alters how much fluid leaves during each pulse, so experimental control requires treating them as coordinated variables rather than relying on pressure alone. This relationship helps produce localized, repeatable deliveries in cell, tissue, and embryo experiments.
Micromanipulation determines where the micropipette tip is positioned, while the pressure pulse determines when and how much fluid is expelled. Keeping these functions distinct allows an investigator to place the tip at a chosen cellular, tissue, or embryonic target and then deliver a controlled volume there. This combination matters when location-specific exposure affects the experimental result.
Precise volume control makes results easier to interpret because differences in cell behavior, gene function, or development can be related to the introduced reagent rather than uncontrolled delivery differences. Pressure injection supports this control by combining adjustable pressure and pulse duration with a fine micropipette. Repeatable delivery is especially valuable when comparing responses among cells, tissues, or embryos.
A basic operation links a reagent-containing micropipette, a pressure source, and a micromanipulator. The operator positions the fine tip at the biological target, applies a brief compressed-air or gas pulse, and uses pressure, pulse duration, and pipette geometry to control the expelled volume. This coordinated workflow supports localized delivery instead of broad exposure across the surrounding sample.
The method can introduce nucleic acids, proteins, dyes, and other reagents through the micropipette. These different cargoes support distinct experimental goals, including examining gene function, tracking cellular behavior, or influencing developmental processes. Because delivery occurs at a selected target, investigators can study responses in specific cells, tissues, or embryos rather than treating the entire preparation uniformly.
Researchers use pressure injection when experiments require localized and repeatable delivery into cells, tissues, or embryos. Applications include embryo manipulation and cellular assays, where introduced nucleic acids, proteins, dyes, or other reagents can support studies of gene function, cell behavior, and development. The technique is particularly useful when both the delivery site and introduced volume must be controlled.