Progesterone
Progesterone - Related Videos
19:26
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
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Cited by 30 •
2013
Intracellular Ca2+ dynamics are very important in sperm physiology and Ca2+-sensitive fluorescent dyes constitute a versatile tool to study them. Population experiments (fluorometry and stopped flow fluorometry) and single cell experiments (flow cytometry and single cell imaging) are used to track spatio-temporal [Ca2+] changes in human sperm cells.
09:01
Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
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Cited by 673 •
2012
Here, we describe how to identify the stage of the murine reproductive (proestrus, estrus, metestrus, or diestrus) by simple, non-invasive collection and cytological assessment of vaginal smear samples. We further describe how vaginal cytology reflects circulating hormonal levels underlying transition through the murine reproductive cycle.
07:38
Techniques for Imaging Ca2+ Signaling in Human Sperm
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Cited by 17 •
2010
Stimulus-evoked [Ca2+]i signals of individual human sperm are assessed. Motile cells are loaded with Ca2+-sensitive fluorescent dye (AM-ester method) and immobilised in a perfusable chamber. Cells are imaged by time-lapse fluorescence microscopy and stimulated via the perfusing medium. Responses of single cells (or regions) are analysed offline using Excel.
09:20
Fertilization of Xenopus oocytes using the Host Transfer Method
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Cited by 13 •
2010
Procedure for fertilizing Xenopus oocytes by the host transfer method.
10:24
Xenopus laevis as a Model to Identify Translation Impairment
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Cited by 2 •
2015
Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.
FAQs
Once progesterone enters a target cell, it binds an intracellular progesterone receptor. This receptor-mediated interaction influences gene transcription, allowing the hormonal signal to produce changes in cellular behavior. The mechanism connects circulating hormone levels with biological effects in reproductive tissues, making receptor activity important for studying endometrial changes, pregnancy support, and reproductive physiology.
Production by the corpus luteum after ovulation places progesterone at a key transition in the reproductive cycle. Its presence helps coordinate post-ovulatory physiology, including preparation of the uterus for possible pregnancy. Later production by the placenta reflects a shift in the tissue supplying the hormone during pregnancy-related reproductive processes.
Progesterone provides one part of the hormonal signaling context within the hypothalamic-pituitary-gonadal axis. Studying its interactions with this axis helps biologists relate ovarian hormone production to reproductive-cycle events and pregnancy-related changes. This perspective is useful because reproductive physiology depends on coordinated signaling among hormone-producing tissues and responsive target cells.
Progesterone activity is associated with preparation of the endometrium, the uterine lining, for pregnancy. That uterine response is biologically distinct from progesterone’s broader role in maintaining early pregnancy and supporting embryonic development. Examining these linked outcomes helps researchers connect receptor-mediated cellular responses with visible changes in reproductive tissues and pregnancy physiology.
In fertility research, progesterone provides a way to examine how post-ovulatory hormone production, uterine preparation, and early pregnancy support are connected. Researchers can use its receptor-mediated effects and its relationship to the reproductive cycle to investigate whether cellular responses align with expected reproductive physiology. This supports broader study of fertility processes without reducing them to a single event.
Progesterone is relevant to hormonal-contraception research because its normal receptor-mediated actions help establish a biological framework for understanding reproductive regulation. Studies can consider how progesterone-related signaling relates to the menstrual cycle, ovulation-related physiology, and uterine conditions. This context allows researchers to examine contraceptive effects alongside the hormone’s natural reproductive functions.
Progesterone research can help clarify reproductive disorders by linking hormone production, intracellular receptor responses, uterine changes, and early pregnancy physiology. Investigators may compare these biological processes across normal reproductive states and disorder-related conditions. The resulting context supports research into how altered progesterone signaling could relate to fertility, cycle regulation, or pregnancy maintenance.