Renal Proximal Tubular Cells
Renal Proximal Tubular Cells - Related Videos
06:32
Development of Human Renal Tubular Epithelial Cell Primary Cultures in Monolayers and Three-Dimensional Conditions
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2025
Here, we describe the protocols for obtaining primary cultures of human renal tubular epithelial cells (HRTEC) from the kidney cortex to develop monolayers and three-dimensional cultures on an extracellular matrix (3D-HRTEC).
06:23
Modeling Hypoxia/Reoxygenation Injury in Proximal Tubular Epithelial Cells
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2025
Here, we describe an in vitro protocol to model hypoxia/reoxygenation damage in the highly metabolic proximal tubular epithelial cells. This model is designed to induce metabolic stress-induced damage, which can be measured through the expression of proximal tubule damage markers and assessment of mitochondrial respiratory function.
03:21
Murine Renal Cell Isolation: A Technique to Isolate and Generate Primary Culture of Renal Tubular Epithelial Cells
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2023
In this video, we demonstrate the steps to isolate and purify renal tubular epithelial cells for a wide range of molecular biology and cell culture studies.
07:06
Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule
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Cited by 3 •
2025
We present an optimized protocol for processing whole human kidneys to isolate and culture primary renal proximal tubule epithelial cells and the application of these cells in a three-dimensional, microfluidic, microphysiological platform to recapitulate the renal proximal tubule.
06:14
Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells
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2025
This article describes the use of confocal microscopy and ImageJ to assess mitochondrial fission/fusion dynamics via Dendra2 photo-switching fluorescence.
FAQs
Transport proteins move selected solutes according to ion gradients, allowing these cells to reclaim glucose, amino acids, bicarbonate, water, and other substances from filtrate. Endocytic pathways provide an additional route for cellular uptake, while secretion transfers selected waste products and medications out of the cells. Together, these mechanisms support precise control of the filtrate’s composition.
Their reabsorptive and secretory work requires high metabolic activity, which can increase sensitivity to inadequate oxygen or other damaging conditions. In addition, these cells encounter compounds carried in the circulation, including medications and potential toxins. This combination makes them useful for investigating how ischemia and chemical exposure disrupt kidney function and produce cellular injury.
Reabsorption returns useful substances from the filtrate to the body, including glucose, amino acids, bicarbonate, water, and other solutes. Secretion moves selected waste products and medications into the tubular contents for removal. Studying both directions of transport helps explain how the proximal tubule preserves essential substances while contributing to clearance of unwanted compounds.
Because these cells are exposed to circulating medications and participate in transport and secretion, they provide a relevant biological context for examining drug-related kidney injury. Research can assess how compounds affect cellular transport, metabolism-related vulnerability, or integrity. Findings may help characterize nephrotoxic effects and support investigation of protective therapies.
Studies can connect altered transport, impaired reabsorption, and cellular injury with disturbances in fluid, electrolyte, or acid-base balance. Their involvement in reclaiming bicarbonate and water makes them relevant to physiological regulation, while their sensitivity to ischemic and toxic stress supports investigation of disease mechanisms. This information can guide studies of potential protective interventions.
They link cellular mechanisms with whole-organ functions because transport proteins, ion gradients, and endocytosis influence the composition of tubular fluid and the recovery of essential substances. Their exposure to circulating compounds also connects normal physiology with toxicology. Consequently, researchers can use them to examine kidney function, transport mechanisms, disease processes, nephrotoxicity, and protective therapies.