Rna-based Processes
Rna-based Processes - Related Videos
10:52
Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
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2026
This protocol describes a visual assay using split Broccoli RNA reporters to detect and quantify intermolecular base pairing in RNA clusters in vitro.
01:32
Pre-mRNA Processing: RNA Splicing
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2023
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
09:12
Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling
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2026
This protocol includes a step-by-step workflow for calcified vascular specimens: tissue handling, decalcification, RNA validation, calcification level detection, and region-of-interest selection strategies on the Nanostring GeoMx Digital Spatial Profiler (DSP). The goal is to present a comprehensive method for preserving vascular tissue morphology and RNA for reliable spatial transcriptomic analysis.
11:00
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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Cited by 105 •
2013
Total cellular RNA provides a poor template for studying short-term changes in RNA synthesis and decay as well as the kinetics of RNA processing. Here, we describe metabolic labeling of newly transcribed RNA with 4-thiouridine followed by thiol-specific biotinylation and purification of newly transcribed RNA allowing to overcome these limitations.
10:59
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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Cited by 1 •
2019
Here, we describe the assembly of RNA polymerase II (Pol II) elongation complexes requiring only short synthetic DNA and RNA oligonucleotides and purified Pol II. These complexes are useful for studying mechanisms underlying co-transcriptional processing of transcripts associated with the Pol II elongation complex.
FAQs
Transcription converts genetic information from DNA into RNA, creating molecules that can convey information for later cellular use. This step links stored genetic information with downstream activities such as RNA processing and translation. Examining transcription helps researchers understand how gene expression begins and how changes in RNA production may relate to cellular function, development, or disease.
RNA splicing and modification expand the study of gene expression beyond the initial production of an RNA molecule. These processing events help explain how RNA is prepared for its cellular roles and why RNA activity cannot be understood from transcription alone. Investigating them provides a framework for examining regulation, biological responses, and differences in cellular function.
Translation uses messenger RNA as the information-bearing molecule for producing protein. This creates a functional connection between RNA sequence information and cellular activities carried out by proteins. In biology, analyzing translation helps clarify how gene expression leads to biological function and how disruptions in this connection may contribute to disease-related changes.
Small and noncoding RNAs can alter gene activity through sequence-specific interactions, allowing regulation to depend on matching or complementary information within cellular molecules. This mechanism distinguishes regulatory RNA from RNA used mainly to convey information for translation. Studying these interactions helps explain dynamic control of gene expression during development and changing cellular conditions.
A useful workflow follows information from DNA transcription into RNA, through RNA processing, and then to messenger RNA translation into protein. Regulatory small or noncoding RNAs can influence gene activity within this sequence of events. Organizing the processes this way helps researchers connect molecular steps with outcomes in development, cellular responses, and disease.
RNA-based processes support gene regulation research, RNA therapeutics, diagnostic testing, and biotechnology. Their dynamic and programmable properties make them useful for investigating how gene activity changes and for developing approaches centered on RNA molecules. These applications connect basic molecular and cellular biology with efforts to understand disease, detect biological states, and influence gene expression.