Root Tissue Harvest
Root Tissue Harvest - Related Videos
12:29
Sterile Tissue Harvest
0 Views •
2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
05:34
A Technique for Harvesting and Dissociating Dorsal Root Ganglia for Neuronal Cultures
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2025
This video demonstrates a method for harvesting neurons from the dorsal root ganglia (DRG). DRG from a rat's spinal column is isolated, treated with enzymes, and then mechanically dissociated to obtain a single-cell suspension. The neurons are separated from glial cells using density gradient centrifugation and cultured in a growth medium.
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
06:06
Apparatus for Harvesting Tissue Microcolumns
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Cited by 7 •
2018
Here we describe a protocol for producing harvesting needles that can be used to collect full-thickness skin tissue without causing donor site scarring. The needles can be combined with a simple collection system to achieve high-volume harvesting.
FAQs
Careful separation protects the validity of downstream measurements. Soil or growth-medium remnants can remain associated with roots, while rough handling may alter the material being examined; either issue can complicate interpretation of structure, composition, or mass. A controlled harvest therefore helps distinguish biological differences from variation introduced during sampling and preparation.
Standardized sampling makes root measurements comparable across treatments, developmental stages, and plant species. If researchers collect or prepare material differently, observed differences may reflect the harvest procedure rather than biology. Consistency is especially important when evaluating root traits alongside environmental conditions, because it supports clearer interpretation of treatment-related changes.
The intended measurement determines how harvested roots should be handled after collection. Imaging requires tissue suitable for examining structure, weighing requires material whose mass can be assessed, while biochemical or molecular assays require preparation compatible with analyzing composition or molecular information. Matching preparation to the endpoint prevents a technically adequate sample from being unsuitable for the planned analysis.
A practical workflow begins by removing roots from soil or another growth medium, then separating root material from surrounding material. The tissue is subsequently preserved or prepared according to the planned measurement, such as imaging, weighing, biochemical analysis, or molecular assays. Keeping these stages distinct helps researchers maintain sample identity and align handling with the study objective.
It can support studies of how roots acquire nutrients, develop over time, interact with microorganisms, and respond to environmental challenges. In stress experiments, harvested material can be examined for changes associated with drought, salinity, or pathogens. Comparing these outcomes across treatments helps connect root-level observations with the conditions imposed on the plant.
A harvested sample can support multiple analytical perspectives when prepared appropriately. Structural imaging can reveal root form, weighing can provide mass-related data, and biochemical or molecular assays can examine composition or molecular responses. These complementary endpoints allow a study to relate visible root traits to underlying biological changes rather than relying on a single measurement.