Saphenous Vein Collection
Saphenous Vein Collection - Related Videos
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
11:35
Decellularization and Recellularization Methodology for Human Saphenous Veins
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Cited by 21 •
2018
Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.
05:12
Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
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Cited by 25 •
2017
Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.
09:50
Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
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Cited by 9 •
2014
The mechanisms leading to the development of intimal hyperplasia (IH) and vein graft failure are still poorly understood. This study describes an ex vivo system to perfuse human veins under controlled flow and pressure. Furthermore the efficiency of external mesh reinforcement to limit the development of IH was evaluated.
00:09
Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg
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2019
Presented here is a protocol for antegrade endoscopic vein harvesting from the lower leg, which can safely be introduced in routine coronary artery bypass grafting. Vein grafts present excellent graft quality following this standardized protocol with positioning of the legs, minimally invasive access to the vein, and antegrade endoscopic vein harvesting.
FAQs
Its superficial position in the lower limb makes the vessel accessible for controlled peripheral blood sampling after the animal is appropriately restrained. Locating this vein allows the operator to direct a sterile lancet or needle to a defined sampling site, supporting consistent collection into a capillary tube or other vessel while limiting unnecessary tissue disruption.
Restraint stabilizes the animal and helps the operator locate the vein accurately. A sterile lancet or needle then creates the puncture needed for blood flow, and the sample is transferred into a capillary tube or collection vessel. Applying pressure afterward promotes hemostasis, meaning control of bleeding, and completes the collection procedure.
The technique can support repeated measurements because it samples peripheral blood without relying on a terminal collection approach. This distinction matters in studies that monitor biological changes over time, such as drug exposure, blood-cell characteristics, plasma biomarkers, or immune responses. Its value is therefore both analytical and practical when longitudinal measurements are required.
Collected blood can support hematology, plasma biomarker analysis, pharmacokinetic assessment, and evaluation of immune responses. These categories allow investigators to examine blood-related characteristics, circulating biological signals, changes associated with compound exposure, and immune activity. The same sampling approach can therefore contribute to several types of biological analysis and monitoring in laboratory rodents.
The described materials include a sterile lancet or needle for puncturing the vein and a capillary tube or collection vessel for receiving the blood. The operator also needs a way to restrain the animal and apply pressure after collection. Together, these components support access, transfer of the sample, and subsequent hemostasis.
A trained operator first restrains the laboratory rodent, identifies the superficial vein in the lower limb, and punctures it with a sterile lancet or needle. Flowing blood is collected into a capillary tube or vessel. Pressure is then applied to the site to achieve hemostasis, completing the sampling workflow.
It is particularly useful when investigators need repeated blood measurements rather than a single terminal sample. Serial collections can support monitoring of hematology, plasma biomarkers, pharmacokinetics, and immune responses. This makes the approach relevant to studies tracking biological or exposure-related changes across multiple observations in laboratory rodents.
The peripheral blood obtained from the saphenous vein provides material for measuring plasma biomarkers, evaluating pharmacokinetics, and assessing immune responses. In this context, the collection method serves as a repeated monitoring tool rather than only a one-time sampling event. It helps connect blood-based measurements with biological changes observed during an experiment.