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Decellularization and Recellularization Methodology for Human Saphenous Veins
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Decellularization and Recellularization Methodology for Human Saphenous Veins

Decellularization and Recellularization Methodology for Human Saphenous Veins

DOI:
10.3791/57803-v

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11:35 min

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July 27, 2018

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, ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg

Chapters

  • 00:04Title
  • 00:43Preparation of Decellularization Setups and Recellularization Bioreactor
  • 03:55Decellularization of Saphenous Vein
  • 06:14Recellularization
  • 09:22Results: Evaluation of Normal, Decellularized, and Recellularized Veins
  • 10:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.

Tags

Keywords: DecellularizationRecellularizationSaphenous VeinTriton-X 100PerfusionWashingBioreactorCell CultureBone MarrowTnBPDNAMedia InletMedia OutletVein InletVein OutletPeristaltic Pump

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