Single Cell Colonies
Single Cell Colonies - Related Videos
02:18
Isolation of Stress-Adapted Bacteria through Single Colony Selection
0 Views •
2025
Source: Jeong, H., et. al. Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat. J. Vis. Exp. (2016).This video demonstrates the selective isolation and growth of stress-adapted bacteria from a culture exposed to prolonged stress conditions. The culture is plated on stressor-containing agar, and a single colony is selected to inoculate a liquid medium with the stressor. Absorbance is measured at regular intervals to monitor the growth and confirm the successful...
08:18
Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
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Cited by 107 •
2010
Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.
13:11
Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
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Cited by 17 •
2010
Video bioinformatics is the automated processing, analysis, understanding, and data mining of biological spatio-temporal data extracted from microscopic videos. The purpose of this article is to demonstrate a method for measuring human embryonic stem cell colony growth using a video bioinformatics method.
09:03
Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample
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2023
Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Seemingly impossible to determine, microbial biodiversity is truly astounding with an estimated one trillion coexisting species (1,2). Although particularly harsh climates, like the acidic environment of the human stomach (3) or the subglacial lakes of Antarctica (4), may be dominated by a specific species, bacteria are typically...
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
FAQs
The founder cell establishes the colony’s defined origin, allowing researchers to relate later cellular behavior to one starting cell rather than to an undefined mixture. This origin is especially important when comparing genetic variation, differentiation, or disease-related phenotypes. It supports more focused interpretation because observed differences can be examined in relation to the colony’s individual cellular source.
Expansion depends on the viability of the isolated cell and the culture environment supporting it. Substrate, nutrient availability, and growth signals can each influence whether repeated cell division continues. These factors therefore affect both colony formation and the reliability of comparisons among colonies, since unsuccessful expansion may reflect culture conditions rather than an intrinsic cellular difference.
Clonal selection examines colonies separately so researchers can identify populations with particular cellular or genetic characteristics. Because each colony has a defined origin, selection can connect a chosen trait with the progenitor-derived population that displays it. This approach is useful when the goal is to distinguish cellular differences that might otherwise be obscured in a broader population.
The workflow begins by obtaining one isolated progenitor cell and placing it under controlled culture conditions. The cell must remain viable, receive suitable nutrients and growth signals, and interact with an appropriate substrate while it undergoes repeated division. Researchers then determine whether the founder cell has expanded into a colony suitable for further biological analysis.
In cell line development, individual colonies provide starting populations with defined cellular origins for selecting and studying desired characteristics. In genetic screening, they help researchers examine variation among progenitor-derived populations rather than treating all cells as equivalent. The resulting comparisons can reveal differences in cellular behavior or traits associated with genetic variation.
Researchers can use these colonies to analyze differentiation, cellular behavior, and disease-related phenotypes. Each colony offers a defined context for asking whether such traits vary among progenitor-derived populations. In biology, this makes the approach valuable for connecting observable outcomes with cellular or genetic differences while preserving the distinction between independently originating colonies.