Skin Sampling
Skin Sampling - Related Videos
05:36
Human Skin Keratinocyte Isolation: A Method to Culture Primary Keratinocytes from Skin Samples
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2023
This video describes the isolation of keratinocytes—the most prominent cells of human skin's epidermal layer, by employing a combination of enzymatic digestion and mechanical disruption methods. This method helps in the preferential growth of keratinocytes over other skin cell types using a selective growth medium.
04:07
Generation of Organotypic Full Skin Reconstructs: A Procedure to Establish 3D Skin Model Using Human Skin Samples
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2023
This video describes the detailed protocol for generating in vitro 3D organotypic skin reconstructs to study melanoma progression. This skin model mimics both the architecture and multicellular complexity of organs in vivo.
04:23
Frozen Skin Tissue Block Preparation: A Protocol to Preserve Cutaneous Melanoma Samples from Murine Dorsal Skin
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2023
This video describes the detailed protocol for isolating melanoma-containing murine dorsal skin, followed by cryo-embedding to prepare a frozen skin tissue block. The cryo-embedded tissue block can be sectioned and microscopically examined to study melanoma progression.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
05:54
Rapid Culturing of Primary Murine Melanocytes and Fibroblasts: Isolating Melanocytes and Fibroblasts from Whole Murine Skin Sample
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2023
This video describes the rapid method to isolate and culture the melanocytes and fibroblast simultaneously from the postnatal mouse pup’s skin. These cells can be maintained and manipulated in vitro to study skin cell biology, wound healing, and melanoma.
FAQs
These variables determine which biological features enter the sample and whether the material reflects the condition being studied. A surface collection may differ substantially from a deeper tissue sample, while timing can affect observations of immune responses, disease-associated changes, or wound healing. Matching collection conditions to the research question improves the accuracy and interpretability of later analyses.
Each location provides access to different biological information. Surface material can support investigation of microbial communities, whereas follicles or deeper tissue may better represent cellular, structural, or disease-associated changes within the skin. Separating these sources helps researchers avoid treating unlike materials as equivalent and allows the chosen analysis to match the tissue compartment of interest.
Preservation conditions influence whether collected material remains suitable for microscopy, histology, molecular assays, or cell culture. A condition that supports one type of analysis may not preserve the features required for another. Researchers therefore need to connect preservation choices with the intended endpoint, because inadequate handling can reduce sample quality and weaken confidence in biological comparisons.
The analytical method determines which aspect of the sample becomes measurable. Microscopy and histology can examine tissue structure, while molecular assays assess gene expression and cell culture supports investigation of viable cells. Using the method that fits the biological question helps distinguish physical tissue changes from molecular activity or cellular behavior in the sampled material.
Planning begins by defining the biological question and selecting a representative site, sampling depth, and collection time. The material is then obtained from the relevant skin compartment, preserved under conditions compatible with the planned assay, and examined using an appropriate analytical approach. Consistency across these steps is important when comparing samples or tracking biological changes.
The approach is useful when investigators need to examine skin structure, microbial communities, immune responses, gene expression, or disease-associated changes. It supports dermatology and infection studies, as well as toxicology and research on wound healing and regeneration. Its value comes from linking a carefully selected tissue source with an analysis that addresses the specific biological process under investigation.