Gfp Rab-3
Gfp Rab-3 - Related Videos
07:59
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Cited by 2 •
2016
We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.
07:25
Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
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Cited by 5 •
2013
A protocol for live imaging of GFP-tagged proteins or autofluorescent structures in individual Drosophila oocytes is described.
07:55
Imaging the Intracellular Trafficking of APP with Photoactivatable GFP
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Cited by 6 •
2015
While the transport of cell surface proteins is relatively easily studied, visualizing the trafficking of intracellular proteins is much more difficult. Here, we use constructs incorporating photoactivatable GFP and demonstrate a method to accurately follow the amyloid precursor protein from the Golgi apparatus to down-stream compartments and follow its clearance.
06:19
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
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Cited by 5 •
2017
This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.
05:28
Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
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Cited by 1 •
2008
The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The...
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The GDP-bound form represents an inactive state, whereas GTP binding activates Rab-3 for regulatory functions at presynaptic terminals. This molecular cycling helps coordinate the transitions required for vesicle docking and fusion. Tracking the tagged protein therefore gives researchers a way to examine how changes in Rab-3 activity may alter the timing or efficiency of neurotransmitter release.
GFP provides a visible signal that allows Rab-3 distribution to be followed in living cells rather than inferred only from biochemical measurements. Changes in fluorescence localization can indicate where Rab-3 is concentrated in relation to synaptic vesicles and presynaptic terminals. This spatial information helps connect protein positioning with regulated secretion and neuronal communication.
Localization places Rab-3 within the cellular sites where synaptic vesicles are handled before neurotransmitter secretion. Observing whether the reporter remains associated with vesicle-rich regions or changes position can help relate trafficking behavior to docking and fusion. As a result, fluorescence patterns provide mechanistic context for understanding how presynaptic organization supports communication between neurons.
The reporter links Rab-3 positioning with release events, allowing researchers to examine trafficking-related localization alongside secretion behavior. A genetic or pharmacological change may modify where Rab-3 appears, how vesicles are positioned, or whether fusion proceeds normally. Comparing these observations helps separate effects on vesicle handling from effects on the release process itself.
A study can express or examine the fluorescently tagged protein in living cells, observe its localization at synaptic or presynaptic regions, and relate those patterns to vesicle release events. Researchers can then compare observations after genetic or pharmacological manipulation. This workflow connects molecular distribution with functional changes in trafficking, docking, fusion, or secretion.
GFP Rab-3 supports broader studies of synaptic vesicle trafficking, neuronal communication, and exocytosis. It can show how Rab-3-associated processes contribute to regulated secretion and how perturbations affect these systems. In biology, the reporter is especially useful when researchers need to connect protein localization with changes in vesicle behavior across living neuronal cells.