Sonication
Sonication - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
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Cited by 5 •
2014
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
FAQs
Acoustic cavitation is the key source of sonication’s biological effects. Sound-driven bubbles expand and collapse in the liquid, producing localized shear forces that can break cellular structures, disperse particles, or fragment nucleic acids. Because these effects occur locally, treatment settings can be adjusted for different sample-processing goals while limiting unnecessary disruption.
Power, treatment duration, temperature, and sample volume are the main conditions requiring optimization. Their combined settings determine whether a sample receives enough acoustic energy for lysis, homogenization, or molecular fragmentation without excessive heating. Researchers therefore adjust these parameters for the specific biological material and intended downstream assay, rather than treating one sonication program as universally suitable.
Acoustic treatment can raise sample temperature, and excessive heating can compromise sensitive biomolecules. Temperature is therefore a core optimization variable alongside power, duration, and volume. Controlling it helps preserve material intended for protein extraction, DNA or chromatin fragmentation, and downstream assays, allowing disruption to occur without sacrificing the molecules needed for analysis.
The biological material is suspended in liquid and exposed to acoustic energy in a sonicator under controlled settings. Researchers optimize power, duration, temperature, and sample volume for the material and objective. After treatment, the processed sample can proceed to lysis-dependent, extraction, homogenization, fragmentation, immunoprecipitation, or sequencing workflows, depending on the preparation goal.
Its uses extend beyond breaking cells to protein extraction, sample homogenization, chromatin fragmentation, and DNA shearing. These applications prepare biological material in forms suitable for subsequent analysis. The intended endpoint determines whether the priority is releasing cellular contents, producing a more uniform sample, or generating fragmented nucleic-acid material for a downstream procedure.
Sonication can prepare samples for downstream immunoprecipitation and sequencing by fragmenting chromatin or DNA before analysis. In these workflows, treatment is not an isolated endpoint: power, duration, temperature, and sample volume must be selected for the intended assay. Optimization therefore links the physical disruption step to the quality of later biological measurements.