Journal
/
Medicine
/
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells

DOI:
10.3791/53060-v

•

09:05 min

•

July 22, 2015

•

, , , ,
1Department of Biology,Syracuse University

Chapters

  • 00:05Title
  • 01:46Cell Sonifier System Cleaning, Setup, and Intensity/Function Assessment
  • 03:30Cell Sonication and Damage Assessment
  • 05:07XTT Assay Assessment of Cell Viability and Mitochondria Activity
  • 06:35Results: Effects of Different Levels of Amplitude on Ultrasonically Lysed U937 and THP1 Cells
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.

Tags

Keywords: Low Frequency UltrasoundNeoplastic CellsCell LysisSonodynamic TherapySonicationSonosensitizersCytoskeletonCholesterol DepletionPreclinical Assessment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53060?article_type=v

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction
check_url/53060?article_type=v

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
check_url/53060?article_type=v

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
check_url/53060?article_type=v

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
check_url/53060?article_type=v

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
check_url/53060?article_type=v

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
check_url/53060?article_type=v

Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
check_url/53060?article_type=v

Quantitative [18F]-Naf-PET-MRI Analysis for the Evaluation of Dynamic Bone Turnover in a Patient with Facetogenic Low Back Pain
check_url/53060?article_type=v

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions
check_url/53060?article_type=v

International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure

Read Article