Splenic Tissue Analysis
Splenic Tissue Analysis - Related Videos
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Induction of Graft Versus Host Disease in Mouse Models via Allogeneic Bone Marrow and Splenic Cell Transplantation
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2025
This video demonstrates the induction of intestinal graft-versus-host disease, GvHD, in a lethally-irradiated recipient mouse using allogeneic hematopoietic stem cells and GvHD-inducing splenic alloreactive T lymphocytes. This protocol helps establish a severe acute intestinal GvHD model to understand the mechanism of the disease and develop innovative therapeutic options.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
09:33
Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
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2026
This protocol successfully prepared sufficient high-quality neovascular tissue from glioma samples using laser-capture microdissection for quantitative proteomics analysis of tumor angiogenesis, overcoming the limitations of studies that traditionally focus mainly on angiogenic factors and providing a large-scale proteomics profile of glioma angiogenesis.
03:42
In Vitro Evaluation of Bacterial Killing by Neonatal Mouse Splenic Phagocytes
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2026
Source: Seman, B. G., et.al. Neonatal Imaging Model of Gram-Negative Bacterial Sepsis. J. Vis. Exp. (2020)This video demonstrates the isolation of neutrophils and inflammatory monocytes from neonatal mouse spleens and their application in a luminescent bacterial killing assay. It covers mechanical dissociation, red blood cell lysis, magnetic bead–based cell enrichment, bacterial incubation, and antibiotic protection. Timed luminescence readings indicate initial bacterial internalization...
08:00
Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
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Cited by 39 •
2011
This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.
FAQs
Examining red and white pulp separately helps connect tissue architecture with immune activity. Differences in their organization, cellular composition, or pathological appearance can indicate altered lymphocyte responses, antigen filtration, inflammation, infection, blood disorders, or tumor involvement. This spatial perspective matters because disease-associated changes may not be distributed uniformly throughout the spleen.
Immunohistochemistry places immune-marker information back into the tissue context. Rather than examining a marker only as an isolated signal, it helps show where that signal occurs relative to splenic architecture and cell populations. This spatial evidence can clarify how lymphocyte responses, inflammation, infection, or tumor involvement are distributed across red and white pulp.
Flow cytometry is useful when analysis needs information about cell populations or immune markers beyond the visual organization of a tissue section. Used alongside histological staining and immunohistochemistry, it broadens the assessment from architecture to cellular characteristics in the sample. This complementarity supports a more complete evaluation of experimental immune responses and disease-related changes.
Comparing healthy and experimental spleens establishes how tissue organization, cell populations, and molecular signals differ under a particular condition. These contrasts can reveal changes associated with immune regulation, disease progression, or treatment response. The comparison is most informative when architectural observations and marker patterns are considered together rather than interpreted as isolated findings.
A typical workflow begins with tissue fixation, followed by sectioning and histological staining to examine structure. Immunohistochemistry can then identify immune markers within the sections. When the study requires additional information about cellular populations or marker patterns, flow cytometry may be included. Together, these stages connect tissue morphology with cellular and molecular observations.
The approach can support investigations of lymphocyte responses, antigen filtration, inflammation, infection, blood disorders, and tumor involvement. Researchers can examine whether these conditions coincide with altered splenic architecture, cell populations, or immune-marker patterns. This makes the analysis useful for linking visible tissue changes with broader questions about immune function and disease biology.
Results provide evidence by combining structural observations with cellular and molecular signals. Differences between samples can indicate changes in immune regulation or pathological involvement, while comparisons across experimental conditions can show patterns associated with disease progression or responses to treatment. Interpreting architecture, cell populations, and immune markers together gives a broader biological picture than any single method alone.