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November 25, 2011
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This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.
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Cite this Article
Gwyther, T. A., Hu, J. Z., Billiar, K. L., Rolle, M. W. Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering. J. Vis. Exp. (57), e3366, doi:10.3791/3366 (2011).
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