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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
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Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

Article DOI: 10.3791/3366
November 25th, 2011 •
1, 1, 1, 1
1Biomedical Engineering Department, Worcester Polytechnic Institute

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November 25th, 2011

This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.

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