Stab Inoculation
Stab Inoculation - Related Videos
07:46
Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
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Cited by 8 •
2015
This article describes a detailed protocol to produce a forebrain stab injury in adult mice. The stab injury induces severe reactive gliosis and glial scar formation which can be subsequently examined by standard immunohistochemistry methods.
09:16
Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration
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Cited by 60 •
2014
To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.
06:09
Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis
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Cited by 2 •
2017
This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
07:57
Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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Cited by 83 •
2011
Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.
FAQs
The location of growth provides a visual clue about motility. If cells remain concentrated along the needle track, the culture shows limited movement through the medium; growth that extends outward from the line indicates that cells have spread. In semisolid media, this contrast makes stab inoculation useful for comparing movement among microorganisms during identification.
A deep medium contains regions with different oxygen availability, so the position and pattern of growth can reveal how a microorganism responds to oxygen conditions. Growth concentrated in a particular part of the medium provides physiological information beyond simple cultivation. This makes the technique valuable when studying oxygen-related traits in biology laboratories.
Medium consistency influences how clearly researchers can observe the inoculation path and any outward spread of cells. In a solid medium, growth can be evaluated in relation to the puncture track, while a semisolid medium can make movement away from that track more apparent. Selecting either format therefore depends on the physiological trait being examined.
A sterile inoculating needle first carries a small sample from a microbial culture. The needle then enters the selected solid or semisolid medium by a puncturing motion, placing cells along the resulting path. After incubation under conditions that support metabolism and reproduction, researchers examine the distribution of growth to assess the culture's behavior.
The basic setup requires a microbial culture, a sterile inoculating needle, and a suitable solid or semisolid growth medium. The medium must support the organism's metabolism and reproduction, while its structure should allow the intended growth pattern to be observed. These choices determine whether the experiment emphasizes cultivation, motility, or oxygen-related physiology.
Researchers use this approach when they need to maintain a culture, support microbial identification, or investigate physiological traits. The resulting growth pattern can show whether cells spread from the inoculation path and where growth occurs within a medium with changing oxygen availability. Such observations connect visible culture behavior with properties relevant to microbial biology.