Steinernema Carpocapsae

Steinernema carpocapsae is an entomopathogenic nematode, a microscopic roundworm that infects and kills insects and serves as a biological control agent in agriculture and biology. Its infective juvenile stage locates susceptible hosts in soil, enters through natural openings, and releases the symbiotic bacterium Xenorhabdus nematophila, which produces toxins and enzymes that rapidly kill the insect. The nematodes feed on the resulting nutrients, develop, reproduce, and release new infective juveniles. Research on S. carpocapsae supports integrated pest management, environmentally compatible control of soil-dwelling and aboveground insect pests, and studies of nematode behavior, symbiosis, and host-pathogen interactions.

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JoVE Journal - Genetics
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CRISPR-Cas9-based Mutagenesis in the Entomopathogenic Nematode Steinernema hermaphroditum and the Maintenance of Mutant Lines

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2025

This article demonstrates CRISPR-Cas9-mediated genome engineering in Steinernema hermaphroditum, an entomopathogenic (EPN: insect-parasitic) nematode and an emerging genetic model. The described technology is useful for creating mutants, allowing for the elucidation of gene functions in nematode biology that are relevant to mutualistic and parasitic symbiosis.

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