Sterile Serological Pipette
Sterile Serological Pipette - Related Videos
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
05:22
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
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2025
Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
FAQs
Graduation marks provide the reference for selecting and checking a milliliter volume before transfer. The user aligns the liquid level with the intended mark, then uses the calibrated barrel to guide aspiration and dispensing. This relationship between marked volume and controlled handling supports consistent measurements, which is especially important when preparing reagents or moving biological samples.
A pipette aid controls the suction and dispensing steps rather than relying on uncontrolled manual movement. It allows liquid to be drawn into the barrel to a selected graduation and then released in a measured way. This controlled pressure helps the operator transfer the intended milliliter volume consistently across repeated biological procedures.
A final blow-out is appropriate when the pipette design requires it. The action expels liquid remaining in the calibrated barrel after the main dispensing step, helping the delivered amount match the intended volume. Because this feature depends on the device design, operators should not apply it automatically when the pipette does not require a blow-out.
Sterility helps minimize contamination during liquid handling, so the pipette can support aseptic media changes, reagent preparation, and sample transfers. This matters because contamination can compromise biological work. Using a sterile device alongside consistent handling protects cultures and contributes to reproducible experiments, linking pipette choice directly to both sample integrity and experimental reliability.
First, select the graduation corresponding to the required milliliter volume. Use the pipette aid to create controlled suction, aspirate liquid to the selected level, and dispense it in a controlled manner. Complete a final blow-out only when the design requires one, maintaining consistent handling throughout the transfer to support accurate and reproducible results.
Their applications include aseptic media changes in cell culture, reagent preparation, and sample transfers in microbiology and molecular biology. In each setting, the combination of graduated measurement, controlled aspiration and dispensing, and sterile handling supports accurate liquid movement. Consistent use can protect cultures and improve reproducibility when experiments require repeated milliliter-scale transfers.