Stress Fibers
Stress Fibers - Related Videos
04:15
Fluorescence Localization of Tight Junction Proteins and Stress Fibers in Endothelial Cells
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2025
Source: Alluri, H., et al. Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction. J. Vis. Exp. (2015).The video demonstrates the fluorescent localization of tight junction proteins and stress fibers in rat brain microvascular endothelial cells (RBMECs). In the stressed monolayer, fluorescently labeled antibodies are used to tag the tight junction proteins, while fluorophore-tagged phalloidin labels the stress...
08:28
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
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Cited by 6 •
2017
A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.
08:42
Tension Test of Fiber-Reinforced Polymeric Materials
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2023
Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA Fiber-reinforced polymeric materials (FRP) are composite materials that are formed by longitudinal fibers embedded in a polymeric resin, thereby creating a polymer matrix with aligned fibers along one or more directions. In its simplest form, the fibers in FRP materials are aligned in an orderly, parallel fashion, thus imparting orthotropic material characteristics, meaning that the material...
03:55
In Vivo Single-Fiber Recording of Sciatic Nerve C-Fibers in Rats
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2025
This video demonstrates a single-fiber recording of C-fiber nerve conduction in rats. An anesthetized rat is incised to expose the sciatic nerve, which provides sensory and motor supply to the lower limbs. The individual C-fibers are then exposed. Electrical stimulation is provided using stimulus electrodes implanted in the foot, and a response is recorded from a single nerve fiber.
11:22
Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
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Cited by 14 •
2014
Described here is a method to directly measure calcium sparks, the elementary units of Ca2+ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca2+ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca2+ signaling can be employed to assess muscle function in health and disease.
FAQs
Nonmuscle myosin II generates tension by sliding actin filaments relative to one another within the bundle. This force-producing activity converts the arrangement of cytoskeletal filaments into mechanical tension that can support cell shape and help the cell respond to physical conditions. Changes in filament organization or myosin activity therefore affect force generation.
Focal adhesions connect cellular force to the extracellular matrix, making them essential sites for transmitting tension beyond the cytoskeleton. Through this linkage, stress-fiber-generated forces contribute to adhesion and spreading and can influence migration. They also provide a physical route through which cells sense and respond to properties of their surroundings.
Mechanical tension generated within these bundles can serve as an input for mechanotransduction, the conversion of mechanical cues into biochemical signals. This links cytoskeletal force production to changes in cell behavior when the physical environment changes. Consequently, stress-fiber organization and dynamics are relevant not only to structure, but also to cellular adaptation.
Organization and dynamics are central readouts: organization describes how the actin-based bundles are arranged, while dynamics concerns how those structures change. Relating these features to tension, focal-adhesion connections, and cell behaviors helps investigators assess how cells maintain shape, spread, migrate, and adjust to physical changes in their environment.
Substrate stiffness provides a physical variable for examining cellular adaptation. Comparing stress-fiber organization and dynamics as stiffness changes can reveal how cells adjust their force-related structures and mechanotransduction responses. This context is especially useful for connecting cytoskeletal behavior with adhesion, spreading, and migration, without treating the cell as mechanically isolated from its surroundings.
These structures are studied in tissue development, wound healing, and cancer invasion because their organization and dynamics help explain how cells generate force, adhere, spread, migrate, and adapt to physical conditions. Examining them can therefore connect cellular mechanics with broader biological processes and disease-related behavior.