Surgical Protocol Mouse
Surgical Protocol Mouse - Related Videos
14:34
Surgical Protocol for a Large, Resealable Cranial Window Enabling Longitudinal, Multi-Modal Electrophysiology Recordings of Mouse Default Mode Network
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2026
This protocol describes a two-phase surgical method to create a large, resealable, dura-sparing cranial window in mice. This technique enables chronic, multi-modal electrophysiological recordings from distributed, deep-brain networks, such as the Default Mode Network, over several weeks.
03:34
Surgical Implantation of Temperature Telemetric Monitoring System in Mouse Model: A Surgical Procedure to Implant Temperature Telemetric Device to Measure Mouse Core Body Temperature
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2025
In this video, we describe a surgical procedure to implant a temperature telemetric device in a mouse model to continuously measure its core body temperature.
04:58
Mouse Ileal Loop Model: A Surgical Model to Study Intestinal Permeability in the Mouse
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2025
This video demonstrates the generation of an ileal loop model to study intestinal permeability in a mouse model. This method provides a live model to understand the epithelial barrier function and consequent immune response.
09:43
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
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Cited by 27 •
2013
Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.
02:53
Neonatal Mouse Ovary Isolation: A Surgical Procedure to Harvest Pair of Ovaries from Neonatal Mouse Model
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2025
In this video, we describe a surgical procedure to isolate ovaries from a neonatal mouse.
FAQs
Anesthesia and physiological monitoring work together to maintain controlled surgical conditions rather than serving as isolated steps. Anesthesia supports the animal during manipulation, while monitoring helps identify changes or complications during the procedure. Recording these conditions allows investigators to distinguish effects associated with the experimental model from variation caused by surgery itself.
Aseptic preparation and careful tissue handling primarily limit avoidable variation during surgery. Consistent preparation helps reduce contamination-related complications, while standardized handling preserves the connection between the surgical manipulation and the biological outcome being measured. This is especially important when the protocol is used to compare animals, experiments, or disease-related conditions.
Postoperative recovery is both an animal-welfare concern and an experimental-quality measure. Observing recovery outcomes can reveal complications that are not apparent during wound closure, and those observations help researchers judge whether animals experienced comparable procedural conditions. Including recovery in the record therefore strengthens interpretation of results rather than treating surgery as complete at closure.
Although the core sequence remains organized, the surgical manipulation and tissue handling must correspond to the biological objective. A disease model may require one type of intervention, whereas injury, transplantation, or organ-function studies may require another. Coordinating the fixed procedural elements with the experimental objective helps preserve comparability without disconnecting the surgery from the question being studied.
Recording anesthesia conditions, aseptic preparation, surgical manipulation, tissue handling, physiological monitoring, wound closure, and postoperative recovery creates a traceable account of the experiment. These records let researchers identify procedural differences, relate recovery outcomes to the intervention, and repeat the work with greater consistency across animals or study periods.
In biology, these procedures support experimental models involving disease, injury, transplantation, and organ function. Their value comes from linking a defined surgical intervention with tissue handling and observed recovery outcomes. This makes the protocol relevant when researchers need to study how a controlled manipulation relates to biological change while maintaining consistent experimental conditions.