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Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
 

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Article doi: 10.3791/50231
January 6th, 2013

Summary January 6th, 2013

Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

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