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Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
JoVE Journal
Medicine
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JoVE Journal Medicine
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

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09:43 min

January 06, 2013

DOI:

09:43 min
January 06, 2013

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Summary

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Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

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