Tissue Specific Isolation
Tissue Specific Isolation - Related Videos
05:25
Dissection and Isolation of Region-Specific Decellularized Lung Tissue
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2023
Presented here is a protocol for the isolation of regional decellularized lung tissue. This protocol provides a powerful tool for studying complexities in the extracellular matrix and cell-matrix interactions.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
10:15
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
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Cited by 20 •
2015
We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.
09:26
Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP
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Cited by 4 •
2016
The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the functions of these regions. This protocol describes procedures to perform engineered DNA-binding molecule-mediated chromatin imunoprecipitation (enChIP) for identification of proteins and RNAs associated with a specific genomic region.
10:10
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
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Cited by 5 •
2010
The molecular basis of spatial-specific phytochrome responses is being investigated using transgenic plants that exhibit tissue- and organ-specific phytochrome deficiencies. The isolation of specific cells exhibiting induced phytochrome chromophore depletion by Fluorescence-Activated Cell Sorting followed by microarray analyses is being utilized to identify genes involved in spatial-specific phytochrome responses.
FAQs
The main tradeoff in tissue-specific isolation is between releasing cells efficiently and preserving their biological state. Mechanical dissociation separates tissue through physical disruption, whereas enzymatic digestion helps reduce extracellular matrix. Excessive processing can affect viability or features needed for analysis or culture, so the selected approach must match the tissue and intended downstream use.
These steps refine the dissociated material in different ways. Filtration helps separate components based on physical passage, centrifugation supports fractionation, and marker-based separation enriches a desired cell population according to its identifying features. Used after dissection and dissociation, they can improve population definition for flow cytometry, molecular assays, or primary culture.
Outcomes depend on the tissue, the balance between mechanical and enzymatic processing, and the degree of downstream enrichment. A protocol may produce many cells but lower purity, or a more enriched population with reduced viability. These tradeoffs matter because morphology, gene expression, culture performance, and assay reliability can all depend on the recovered cell population.
A typical workflow begins with tissue dissection, followed by mechanical dissociation, enzymatic digestion, or a combination of both. The resulting material can then undergo filtration, centrifugation, or marker-based separation to enrich the desired population. The isolated cells are subsequently directed into an application such as primary culture, flow cytometry, or molecular analysis.
Researchers use these cells when cellular behavior must be connected to tissue of origin. The material can support studies of cell morphology, gene expression, development, and disease, while primary culture enables observation or further experimentation outside the original tissue. The same preparation may also contribute to tissue engineering or population-based analyses.
Because the cells remain linked to a defined tissue source, their morphology and gene expression can be interpreted in relation to that biological context. Enrichment through filtration, centrifugation, or marker-based separation can help focus analysis on a desired population. This supports comparisons in development and disease, provided yield, purity, and viability are adequate.