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Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
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Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

DOI:
10.3791/1925-v

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10:10 min

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May 29, 2010

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,
1Department of Energy – Plant Research Laboratory,Michigan State University (MSU), 2Department of Biochemistry and Molecular Biology,Michigan State University (MSU)

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:22Producing the GAL4 Enhancer-trap Line
  • 02:37Plant Growth and Leaf Protoplasts Isolation
  • 07:18Protoplast Sorting by Fluorescence-Activated Cell Sorting (FACS)
  • 08:47Leaf Protoplasts Isolation and Sorting by FACS Representative Results
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

The molecular basis of spatial-specific phytochrome responses is being investigated using transgenic plants that exhibit tissue- and organ-specific phytochrome deficiencies. The isolation of specific cells exhibiting induced phytochrome chromophore depletion by Fluorescence-Activated Cell Sorting followed by microarray analyses is being utilized to identify genes involved in spatial-specific phytochrome responses.

Tags

FACS-assisted Cell-type Specific Expression ProfilingArabidopsis ThalianaPhotoreceptorsRed/far-red Light-absorbing PhytochromePlant SystemsPhytochrome FunctionsMutational AnalysesPhotoperceptionPhotomorphogenesisDownstream Target GenesCell-to-cell Signaling

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