Tomato Leaf Curl
Tomato Leaf Curl - Related Videos
02:11
Curli Fiber-Mediated Stabilization of Bacterial Hydrogel Patterns
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2026
Source: Spiesz, E. M., et al., Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter. J. Vis. Exp. (2019)This video demonstrates a method for comparing 3D-printed alginate patterns containing either control or genetically engineered bacterial strains. The test strain produces curli fibers upon induction, which reinforce the alginate hydrogel structure. Following treatment with a calcium-chelating solution, only the test pattern remains intact, highlighting the...
15:28
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
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Cited by 3 •
2012
The design of a synthetic operon encoding both the secretory apparatus and the structural monomers of curli fibers is described. Overproduction of these amyloids and adherent polymers allows a measurable gain of adherence of the E. coli chassis1. Easy ways to visualize and quantify adherence are explained.
02:30
Seedling Flood Assay for Screening Bacterial Resistance in Tomatoes
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2025
Source: Hassan, J. A., et al. High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay. J. Vis. Exp. (2020).This video demonstrates a method to screen for bacterial resistance in different tomato strains using ten-day-old seedlings inoculated with a bacterial suspension. It explains how bacteria invade through stomata, deliver effector proteins to suppress plant immunity, and how plant responses differ between susceptible and resistant...
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Cited by 159 •
2009
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
06:41
High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
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Cited by 2 •
2020
The seedling flood assay facilitates rapid screening of wild tomato accessions for the resistance to the Pseudomonas syringae bacterium. This assay, used in conjunction with the seedling bacterial growth assay, can assist in further characterizing the underlying resistance to the bacterium, and can be used to screen mapping populations to determine the genetic basis of resistance.
FAQs
Begomoviruses can disrupt host gene regulation and normal development, altering how tomato leaves expand and maintain their form. This mechanism helps explain why affected foliage may become distorted rather than showing only generalized damage. Studying these virus-driven changes provides insight into plant-virus interactions and helps connect visible symptoms with underlying biological processes.
Whiteflies spread the disease-causing viruses while feeding, moving them from infected tomato plants to healthy ones. Their presence therefore provides an important epidemiological clue when symptoms appear in a crop. Monitoring the vector is relevant not only for understanding transmission but also for planning management strategies aimed at reducing virus movement between plants.
Leaf curling and reduced expansion do not identify a single cause by themselves. Begomovirus infection can produce this syndrome, but nutrient problems, water stress, and herbicide injury may create similar visual patterns. Researchers therefore interpret symptoms alongside whitefly presence and molecular test results, reducing the risk of attributing environmentally caused damage to an infection.
Investigation commonly combines three types of evidence: symptom patterns, observations of the whitefly vector, and molecular testing. Symptoms indicate the affected developmental processes, vector evidence supports a possible transmission route, and molecular tests help assess whether a virus is present. Using these sources together improves differentiation from nutrient, water, or herbicide injury.
Surveillance can track symptom patterns, identify possible vector-associated spread, and support molecular confirmation of suspected infections. These observations help researchers and growers recognize disease activity and respond with informed crop-protection measures. The broader value lies in connecting field diagnosis with resistant cultivar selection and vector management rather than relying on symptoms alone.
The disorder provides a model for examining how plant viruses alter host gene regulation and development. It also links molecular mechanisms with vector-mediated transmission and visible changes in crop growth. This combination makes the system useful for studying plant-virus interactions while informing practical goals such as disease surveillance, resistant cultivar selection, and crop protection.