Transwell Culture System
Transwell Culture System - Related Videos
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
03:20
A Transwell Tissue Model for Studying Interactions Between Human Oral Biofilms and Epithelium
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2026
Source: Alshehri, M. et. al., Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro. J. Vis. Exp. (2025)This video demonstrates a co-culture model using multi-species oral biofilms and human oral epithelium. It highlights epithelial immune activation and enables the downstream analysis of host-pathogen interaction.
03:05
Fluorescence Based Transwell Macrophage Attraction Assay: An In Vitro Method to Study Tumor-Macrophage Interaction Through Chemical-Induced Chemotaxis
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2023
In this video, we study tumor-macrophage interaction using fluorescence-based transwell macrophage interaction assay. The assay involves the analysis of the migration of macrophages through chemical-induced chemotaxis.
07:41
Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
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Cited by 13 •
2012
Interstitial fluid flow is elevated in solid tumors and can modulate tumor cell invasion. Here we describe a technique to apply interstitial fluid flow to cells embedded in a matrix and then measure its effects on cell invasion. This technique can be easily adapted to study other systems.
02:52
A Technique to Establish a Bacterial Infection Model in an Ex Vivo Organ Culture
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2025
This video demonstrates a method to establish an infection model in placental tissues to study infection propagation. An ex vivo placental organ culture is infected with Listeria monocytogenes, a facultative intracellular parasite. The bacteria invade extravillous trophoblasts (EVTs) to enter the placental tissue and then propagate to other cells via cell-to-cell spread.
FAQs
Pore size determines whether an experiment primarily measures exchange of soluble factors or also permits migration through the membrane. Smaller or more restrictive pores can support compartmentalized culture and diffusion, whereas pores suitable for passage allow cells to move between compartments. Selecting the pore size therefore links the physical setup to the biological question and measured outcome.
In chemotaxis studies, the two compartments establish a spatially separated environment in which soluble cues influence directional cell movement. The membrane provides a route for migration while retaining the upper and lower organization needed to compare movement toward or away from a stimulus. This arrangement helps investigators examine how cells respond to biochemical signals.
For epithelial or endothelial barrier experiments, the membrane serves as a surface on which a cell layer can be evaluated while the compartments remain distinct. Changes in the movement of soluble substances across the system can be interpreted as differences in membrane permeability or barrier function. The design connects cellular organization with measurable transport behavior.
Placing cells on one side, both sides, or in separate populations allows investigators to vary which cell types occupy the system while preserving communication through diffusible factors. This flexibility helps distinguish effects produced by a single population from effects arising during intercellular signaling. It is especially relevant when studying interactions between different cell populations.
A basic workflow begins by selecting a membrane pore size and deciding whether cells will be cultured on one side or both. Cells are then maintained in the separated compartments under controlled conditions, after which investigators assess migration, permeability, barrier behavior, or communication. The selected readout should match what the membrane configuration permits.
Researchers apply these systems to questions that require controlled separation without eliminating biochemical exchange. In biology, applications include chemotaxis, epithelial and endothelial barriers, inflammation, cancer invasion, and communication between cell populations. Results can indicate whether a response involves migration across the membrane, altered permeability, or signals released by cells in another compartment.